通过聚合酶链反应检测宫颈管标本中的沙眼衣原体。
Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction.
作者信息
Loeffelholz M J, Lewinski C A, Silver S R, Purohit A P, Herman S A, Buonagurio D A, Dragon E A
机构信息
Roche Molecular Systems, Branchburg, New Jersey 08876-1760.
出版信息
J Clin Microbiol. 1992 Nov;30(11):2847-51. doi: 10.1128/jcm.30.11.2847-2851.1992.
Abstract
A rapid and sensitive polymerase chain reaction (PCR)-based assay for detection of Chlamydia trachomatis in cervical specimens is described. This assay consists of (i) sample preparation which avoids the use of heat, centrifugation, or organic extractions; (ii) rapid, two-temperature PCR amplification of C. trachomatis cryptic plasmid sequences; and (iii) capture and colorimetric detection of amplified DNA in microwell plates. PCR was compared with culture by using 503 cervical specimens. After resolution of discrepant specimens with a confirmatory PCR assay directed against the chlamydial major outer membrane protein gene, PCR had a sensitivity of 97% and a specificity of 99.7% while culture had a sensitivity of 85.7% and a specificity of 100%. In a separate study, PCR was compared with a direct specimen enzyme immunoassay (Chlamydiazyme; Abbott Diagnostics) by using 375 cervical specimens. After resolution of discrepant specimens, PCR had a sensitivity and specificity of 100%, while the enzyme immunoassay had a sensitivity of 58.8% and a specificity of 100%.
摘要
本文描述了一种基于聚合酶链反应（PCR）的快速灵敏检测宫颈标本中沙眼衣原体的方法。该方法包括：（i）样品制备，避免使用加热、离心或有机萃取；（ii）对沙眼衣原体隐蔽质粒序列进行快速双温PCR扩增；（iii）在微孔板中捕获并比色检测扩增的DNA。通过使用503份宫颈标本，将PCR与培养法进行比较。在用针对衣原体主要外膜蛋白基因的验证性PCR检测解决了有差异的标本后，PCR的灵敏度为97%，特异性为99.7%，而培养法的灵敏度为85.7%，特异性为100%。在另一项研究中，通过使用375份宫颈标本，将PCR与直接标本酶免疫测定法（衣原体酶免疫测定；雅培诊断公司）进行比较。在解决了有差异的标本后，PCR的灵敏度和特异性均为100%，而酶免疫测定法的灵敏度为58.8%，特异性为100%。
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本文引用的文献
1
Comparison of three techniques for detection of Chlamydia trachomatis in endocervical specimens from asymptomatic women.三种检测无症状女性宫颈标本中沙眼衣原体方法的比较。
J Clin Microbiol. 1988 Apr;26(4):726-31. doi: 10.1128/jcm.26.4.726-731.1988.
2
Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication.沙眼衣原体L1血清型隐蔽质粒全核苷酸序列分析。参与DNA复制的证据。
Nucleic Acids Res. 1988 May 11;16(9):4053-67. doi: 10.1093/nar/16.9.4053.
3
Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction.利用聚合酶链反应对沙眼衣原体所有血清型共有的DNA序列进行特异性扩增。
Res Microbiol. 1989 Jan;140(1):7-16. doi: 10.1016/0923-2508(89)90053-3.
4
Laboratory diagnosis of human chlamydial infections.人类衣原体感染的实验室诊断
Clin Microbiol Rev. 1989 Apr;2(2):119-36. doi: 10.1128/CMR.2.2.119.
5
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Plasmid. 1990 Mar;23(2):144-8. doi: 10.1016/0147-619x(90)90033-9.
6
Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay.通过酶免疫测定法检测扩增的DNA来诊断沙眼衣原体宫颈感染。
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7
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J Clin Microbiol. 1990 Jun;28(6):1254-60. doi: 10.1128/jcm.28.6.1254-1260.1990.
8
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Plasmid. 1990 Mar;23(2):149-54. doi: 10.1016/0147-619x(90)90034-a.
9
Comparison of a DNA probe with culture for detecting Chlamydia trachomatis directly from genital specimens.
Mol Cell Probes. 1990 Feb;4(1):25-31. doi: 10.1016/0890-8508(90)90036-y.
10
Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study.一项随访研究中确定的聚合酶链反应用于衣原体检测的诊断价值。
J Clin Microbiol. 1991 Jan;29(1):42-5. doi: 10.1128/jcm.29.1.42-45.1991.
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