Goldfarb Allan H, McKenzie Michael J, Bloomer Richard J
Department of Exercise and Sport Science, Room 250 HHP Building, University of North Carolina Greensboro, Greensboro, NC 27402-6170, USA.
Appl Physiol Nutr Metab. 2007 Dec;32(6):1124-31. doi: 10.1139/H07-078.
The purpose of this study was to determine the influence of gender and antioxidant supplementation on exercise-induced oxidative stress. Twenty-five men and 23 women ran for 30 min at 80% VO2 max, once before and once after 2 weeks of supplementation, and again after a 1-week wash-out period. Subjects were randomly assigned to either placebo (P), antioxidant (A: 400 IU vitamin E+1 g vitamin C), or a fruit and vegetable powder (FV) treatment. Blood was obtained at rest and immediately after exercise. Before supplementation, women had higher resting reduced glutathione, total glutathione, and plasma vitamin E compared with men. With both A and FV supplementations, plasma vitamin E gender differences disappeared. Protein carbonyls, oxidized glutathione, and malondialdehyde all increased similarly for both genders in response to exercise. Both A and FV attenuated the reduced glutathione decrease and the oxidized glutathione and protein carbonyls increase compared with P, with no gender differences. 8-hydroxydeoxyguanosine was lower with treatment A compared with FV and P only for men. Plasma vitamin C increased 39% (A) and 21% (FV) compared with P. These data indicate that women have higher resting antioxidant levels than men. Markers of oxidative stress increased similarly in both genders in response to exercise of similar intensity and duration. Two weeks of antioxidant supplementation can attenuate exercise-induced oxidative stress equally in both genders.
本研究的目的是确定性别和抗氧化剂补充对运动诱导的氧化应激的影响。25名男性和23名女性以80%最大摄氧量跑30分钟，在补充2周前、补充2周后以及1周洗脱期后各进行一次。受试者被随机分配到安慰剂（P）、抗氧化剂（A：400 IU维生素E + 1 g维生素C）或果蔬粉（FV）治疗组。在静息状态和运动后立即采集血液。补充前，女性静息状态下的还原型谷胱甘肽、总谷胱甘肽和血浆维生素E水平高于男性。补充A和FV后，血浆维生素E的性别差异消失。运动后，蛋白质羰基、氧化型谷胱甘肽和丙二醛在两性中均有相似程度的增加。与P组相比，A和FV均减轻了还原型谷胱甘肽的减少以及氧化型谷胱甘肽和蛋白质羰基的增加，且无性别差异。仅在男性中，与FV和P组相比，治疗A组的8-羟基脱氧鸟苷水平较低。与P组相比，血浆维生素C在A组增加了39%，在FV组增加了21%。这些数据表明，女性静息状态下的抗氧化剂水平高于男性。在进行相似强度和时长的运动后，两性的氧化应激标志物增加程度相似。为期两周的抗氧化剂补充可同等程度减轻两性运动诱导的氧化应激。
