Binder Wolfgang H, Sachsenhofer Robert, Farnik Dominique, Blaas Dieter
Martin-Luther University Halle-Wittenberg, Faculty of Natural Science II (Chemistry and Physics), Institute of Chemistry/Macromolecular Chemistry, Heinrich-Damerowstr. 4/TGZ III, D-06120, Halle, Saale, Germany.
Phys Chem Chem Phys. 2007 Dec 28;9(48):6435-41. doi: 10.1039/b711470m. Epub 2007 Oct 29.
The present paper investigates the selective incorporation of preformed nanoparticles (hydrophobic Au-NP (2 nm); hydrophilic Au-NP (12 nm); hydrophobic CdSe-NP (1.9 nm); retrovirus-particles (approximately 30 nm)) into the interface of lipid vesicles and polymersomes via TEM and DLS investigations. Lipid membranes were made from N,N-dimethyl-N,N-dioctadecylammonium bromide (DODAB), di-oleoyl-phosphatidylcholine (DOPC), whereas polymersome-membranes were fabricated from the diblock copolymer poly-(butadiene-block-ethylenoxide). Stabilization of the final structures was achieved via sol/gel processes at the outside of the membranes, thus stabilizing the structure by a silicate shell. Whereas hydrophobic Au-NPs can be successfully embedded into the polymersome- and lipid-vesicle membranes, hydrophilic nanoparticles were found evenly distributed in the inner- and outer compartments of the vesicles and polymersomes. Significant effects such as size reduction, selective enrichment of all nanoparticles within only few polymersomes as well as budding effects of larger entities (i.e., viral particles) are described.
本文通过透射电子显微镜（TEM）和动态光散射（DLS）研究，探究了预制纳米颗粒（疏水性金纳米颗粒（2纳米）；亲水性金纳米颗粒（12纳米）；疏水性硒化镉纳米颗粒（1.9纳米）；逆转录病毒颗粒（约30纳米））选择性掺入脂质囊泡和聚合物囊泡界面的情况。脂质膜由N，N-二甲基-N，N-二辛基溴化铵（DODAB）、二油酰磷脂酰胆碱（DOPC）制成，而聚合物囊泡膜则由二嵌段共聚物聚（丁二烯-嵌段-环氧乙烷）制成。通过膜外部的溶胶/凝胶过程实现最终结构的稳定，从而通过硅酸盐壳稳定结构。疏水性金纳米颗粒能够成功嵌入聚合物囊泡和脂质囊泡膜中，而亲水性纳米颗粒则均匀分布在囊泡和聚合物囊泡的内部和外部隔室中。文中描述了诸如尺寸减小、所有纳米颗粒仅在少数聚合物囊泡中选择性富集以及较大实体（即病毒颗粒）的出芽效应等显著影响。
