Deike Stefanie, Malke Marlen, Lechner Bob-Dan, Binder Wolfgang H
Faculty of Natural Science II, Martin Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, 06120 Halle (Saale), Germany.
School of Physics, University of Exeter, Stocker Road, Exeter EX4, UK.
Polymers (Basel). 2017 Aug 17;9(8):369. doi: 10.3390/polym9080369.
Investigation of model biomembranes and their interactions with natural or synthetic macromolecules are of great interest to design membrane systems with specific properties such as drug-delivery. Here we study the behavior of amphiphilic β-turn mimetic polymer conjugates at the air⁻water interface and their interactions with lipid model membranes. For this endeavor we synthesized two different types of conjugates containing either hydrophobic polyisobutylene (PIB, = 5000 g·mol) or helical poly(-hexyl isocyanate) (PHIC, = 4000 g·mol), both polymers being immiscible, whereas polyisobutylene as a hydrophobic polymer can incorporate into lipid membranes. The conjugates were investigated using Langmuir-film techniques coupled with epifluorescence microscopy and AFM (Atomic Force Microscopy), in addition to their phase behavior in mixed lipid/polymer membranes composed of DPPC (dipalmitoyl--glycero-3-phosphocholine). It was found that the DPPC monolayers are strongly disturbed by the presence of the polymer conjugates and that domain formation of the polymer conjugates occurs at high surface pressures (π > 30 mN·m).
研究模型生物膜及其与天然或合成大分子的相互作用对于设计具有特定性质（如药物递送）的膜系统具有重要意义。在此，我们研究两亲性β-转角模拟聚合物缀合物在气-水界面的行为及其与脂质模型膜的相互作用。为此，我们合成了两种不同类型的缀合物，一种含有疏水性聚异丁烯（PIB，Mw = 5000 g·mol），另一种含有螺旋状聚（己基异氰酸酯）（PHIC，Mw = 4000 g·mol），这两种聚合物互不相溶，而聚异丁烯作为疏水性聚合物可以掺入脂质膜中。除了在由二棕榈酰磷脂酰胆碱（DPPC）组成的混合脂质/聚合物膜中的相行为外，还使用Langmuir膜技术结合落射荧光显微镜和原子力显微镜（AFM）对缀合物进行了研究。结果发现，聚合物缀合物的存在会强烈干扰DPPC单层，并且聚合物缀合物在高表面压力（π > 30 mN·m）下会形成域。
