Masuda Susumu, Yamaguchi Hitomi, Kurokawa Toshiko, Shirakami Tomoyuki, Tsuji Ryohei F, Nishimura Ikuko
Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba, 278-0037, Japan.
Int J Food Microbiol. 2008 Feb 10;121(3):245-52. doi: 10.1016/j.ijfoodmicro.2007.10.011. Epub 2007 Nov 1.
A halophilic lactic acid bacterium, Tetragenococcus halophilus, was found to possess an immunomodulatory activity that promotes T helper type 1 (Th1) immunity in addition to its important roles in soy sauce brewing. Strain Th221 was selected from 151 strains isolated from soy sauce (shoyu) moromi, since it induced strong interleukin (IL)-12 production by mouse peritoneal macrophages in vitro. The relationship between the salt concentration in the medium and the IL-12 production-inducing activity of this strain was investigated, and the activity was found to be strong when the bacteria were grown in medium containing > or =10% (w/v) salt. The Th1-promoting activity was also manifested in an in vivo mouse study, since Th1-dependant contact sensitivity was augmented and Th2 immunity, as evaluated by specific immunoglobulin E production, was suppressed following oral ingestion of Th221. Based on these findings, Th221 administration may be useful for improving allergic symptoms.
一种嗜盐乳酸菌——嗜盐四联球菌，除了在酱油酿造中发挥重要作用外，还被发现具有促进1型辅助性T细胞（Th1）免疫的免疫调节活性。从151株从酱油醪中分离出的菌株中挑选出Th221菌株，因为它在体外能诱导小鼠腹腔巨噬细胞产生强烈的白细胞介素（IL）-12。研究了培养基中盐浓度与该菌株诱导IL-12产生活性之间的关系，发现当细菌在含≥10%（w/v）盐的培养基中生长时，该活性较强。在一项体内小鼠研究中也表现出了Th1促进活性，因为口服Th221后，Th1依赖的接触敏感性增强，而通过特异性免疫球蛋白E产生评估的Th2免疫受到抑制。基于这些发现，给予Th221可能有助于改善过敏症状。
