Murthy R C, Saxena D K, Gupta S K, Chandra S V
Neurotoxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
Reprod Toxicol. 1991;5(5):443-7. doi: 10.1016/0890-6238(91)90008-4.
The administration of hexavalent chromium (2 mg/kg, ip as potassium dichromate) in adult rats daily for 15 days produced significant increases in the blood and testicular chromium levels. Although no light microscopic pathologic changes or alterations in epididymal sperm counts and motility were observed, lanthanum perfusion in treated rats revealed leakage of Sertoli-cell tight junctions under EM. A few tubules showed marked ultracellular alterations in the form of vacuolization of cytoplasm and degeneration of mitochondria in the epithelial cells. Late stage spermatids were the most affected germ cells. The mitochondrial sheath of the midpiece was vacuolated, incomplete, swollen, or broken in places. The observed alterations may result in the disruption of normal testicular physiology leading to reproductive impairment after chromium exposure.
成年大鼠每天腹腔注射六价铬（以重铬酸钾形式，2毫克/千克），持续15天，会使血液和睾丸中的铬水平显著升高。尽管在光学显微镜下未观察到病理变化，附睾精子计数和活力也未改变，但对处理过的大鼠进行镧灌注后，电镜观察显示支持细胞紧密连接有渗漏。少数曲细精管显示出明显的超微结构改变，表现为上皮细胞胞质空泡化和线粒体变性。晚期精子细胞是受影响最严重的生殖细胞。中段的线粒体鞘有空泡化、不完整、肿胀或局部破裂的情况。观察到的这些改变可能导致睾丸正常生理功能紊乱，从而在铬暴露后导致生殖功能受损。
