Pohanka M, Skládal P
Center of Biological Defense, 561 66 Tĕchonín, Czechia.
Folia Microbiol (Praha). 2007;52(4):325-30. doi: 10.1007/BF02932086.
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detect F. tularensis with the limit of detection 10(5) CFU/mL with a typical measuring cycle > 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
开发了一种基于压电传感器的新型免疫传感装置，用于直接检测生物战剂土拉弗朗西斯菌。该传感器包括固定在与传感器金电极共价连接的蛋白A层中的小鼠多克隆抗体。该免疫传感器能够检测土拉弗朗西斯菌，检测限为10(5) CFU/mL，典型测量周期>5分钟。该传感器已成功用于快速检测饮用水和牛奶中的土拉弗朗西斯菌加标；与缓冲溶液相比，未观察到灵敏度下降。所提出的快速测量微生物制剂的概念对于短时间预培养富集后的样品评估似乎很有前景。
