Spehar-Délèze Anna-Maria, Julich Sandra, Gransee Rainer, Tomaso Herbert, Dulay Samuel B, O'Sullivan Ciara K
Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007, Tarragona, Spain.
Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffer-Institut, Naumburger Str. 96A, 07743, Jena, Germany.
Anal Bioanal Chem. 2016 Oct;408(25):7147-53. doi: 10.1007/s00216-016-9658-x. Epub 2016 Jun 2.
An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 (2+)-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.
描述了一种电化学发光（ECL）免疫传感器，该传感器使用全抗体或抗体片段作为捕获生物分子来快速检测土拉弗朗西斯菌病原体。采用夹心免疫分析法，以脂多糖（LPS）或全灭活细菌细胞（LVS）为靶标，同时将钌（联吡啶）3（2+）封装的硅酸盐纳米颗粒连接到二抗上并用作ECL标记物。该分析在一个定制的黑匣子中的微流控芯片中进行，该黑匣子集成了电子学、光学和流体ics。当捕获分子为全抗体时，LPS的检测限为0.4 ng/mL，而当捕获分子为抗体F（ab）片段时，LVS的检测限分别为70和45个细菌/mL。
