用于检测和鉴定土拉弗朗西斯菌的单克隆抗体的制备。
Preparation of monoclonal antibodies for detection and identification of Francisella tularensis.
作者信息
Hotta Akitoyo, Uda Akihiko, Fujita Osamu, Tanabayashi Kiyoshi, Yamada Akio
机构信息
Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Tokyo 162-8640, Japan.
出版信息
Clin Vaccine Immunol. 2007 Jan;14(1):81-4. doi: 10.1128/CVI.00057-06. Epub 2006 Nov 22.
Abstract
Monoclonal antibodies (MAbs) against Francisella tularensis were obtained. Three MAbs specifically reacted with F. tularensis, while four MAbs reacted with other members of the genus Francisella as well. Fluorescent isothiocyanate-conjugated MAbs unequivocally stained bacterial cells in specimens from experimentally infected mice. Two MAbs agglutinated F. tularensis antigen in the agglutination tests. These MAbs should improve methods for detection and identification of F. tularensis.
摘要
获得了针对土拉弗朗西斯菌的单克隆抗体（MAbs）。三种单克隆抗体与土拉弗朗西斯菌特异性反应，而另外四种单克隆抗体也与弗朗西斯菌属的其他成员反应。异硫氰酸荧光素偶联的单克隆抗体明确地对实验感染小鼠标本中的细菌细胞进行了染色。两种单克隆抗体在凝集试验中凝集了土拉弗朗西斯菌抗原。这些单克隆抗体应能改进土拉弗朗西斯菌的检测和鉴定方法。
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