Pohanka Miroslav, Treml František, Hubálek Martin, Banďouchová Hana, Beklová Miroslava, Pikula Jiří
Center of Biological Defense, Těchonín, Czech Republic.
Department of Infectious Diseases and Epizootiology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Sensors (Basel). 2007 Nov 19;7(11):2825-2834. doi: 10.3390/s7112825.
Piezoelectric biosensor was used for diagnosis of infection by Francisellatularensis subsp. holarctica in European brown hares. Two kinds of experiments wereperformed in this study. First, sera from experimentally infected European brown hares(Lepus europaeus) were assayed by piezoelectric biosensor and the seventh day postinfection was found as the first one when statistically significant diagnosis of tularemia waspossible; all other sera collected from hares later than on day 7 following the infection werefound tularemia positive. Typing to classify the field strain of F. tularensis used for theexperimental infection was confirmed by proteome study. Second, sera from 35 Europeanbrown hare specimens sampled at hunting grounds and tested as tularemia positive by slowagglutination allowed diagnosis of tularemia by the piezoelectric biosensor. All these sera ofnaturally infected hares were found as tularemia positive, too. Efficacy of the piezoelectricbiosensor for the serological diagnosis of tularemia is discussed.
压电生物传感器用于诊断欧洲棕兔感染土拉热弗朗西斯菌全北区亚种。本研究进行了两种实验。首先，用压电生物传感器检测实验感染的欧洲棕兔（欧洲野兔）的血清，发现感染后第7天是首次能够进行兔热病统计学显著诊断的时间；从感染后第7天以后采集的所有其他兔血清均为兔热病阳性。通过蛋白质组学研究证实了用于实验感染的土拉热弗朗西斯菌田间菌株的分型。其次，对在狩猎场采集的35份欧洲棕兔样本的血清进行检测，这些血清通过慢速凝集试验检测为兔热病阳性，压电生物传感器能够诊断出兔热病。所有这些自然感染野兔的血清也均为兔热病阳性。本文讨论了压电生物传感器用于兔热病血清学诊断的有效性。
