Agrawal S C
Department of Botany, University of Allahabad, Allahabad 211 002, India.
Folia Microbiol (Praha). 2007;52(4):399-406. doi: 10.1007/BF02932095.
Batch cultures of Chlorella vulgaris and C. variegata reproducing about twice every 5 d within 0-15 d had vegetative cells and autospore mother cells in the ratio of about 19 : 1. Continuous slow or negligible and/or no growth in > 15-d-old control cultures or in young cultures supplied with the antibiotics streptomycin, penicillin, amoxycillin (10-1000 ppm) or tetracycline (10, 100 ppm), and pesticides carbofuran, gammaxine, moticop or iralon (1-100 ppm) was due to slow autospore mother cells dehiscence (leading to an increase in their percentage); while negligible and/or no growth of both algal species in sewage water (100, 25%), detergent (0.1-1%), petrol or kerosene (5-20 %), benzene, toluene or phenol (5, 10%) and pesticides rogor or endosulfan (1, 10 ppm) was due to vegetative cells failure to differentiate into auto-spore mother cells (leading to decreased/zero autospore mother cells percentage) and/or rapid death of all cells. C. variegata was equally or slightly more sensitive to different chemical stress than C. vulgaris.
在0至15天内每5天繁殖约两代的普通小球藻和杂色小球藻分批培养物中,营养细胞与自孢子母细胞的比例约为19:1。在超过15天的对照培养物中,或在添加抗生素链霉素、青霉素、阿莫西林(10 - 1000 ppm)或四环素(10、100 ppm)以及农药克百威、六六六、莫替考普或艾拉酮(1 - 100 ppm)的年轻培养物中,持续缓慢生长或生长可忽略不计和/或不生长,是由于自孢子母细胞开裂缓慢(导致其百分比增加);而在污水(100、25%)、洗涤剂(0.1 - 1%)、汽油或煤油(5 - 20%)、苯、甲苯或苯酚(5、10%)以及农药乐果或硫丹(1、10 ppm)中,两种藻类生长可忽略不计和/或不生长,是由于营养细胞无法分化为自孢子母细胞(导致自孢子母细胞百分比降低/为零)和/或所有细胞迅速死亡。杂色小球藻对不同化学胁迫的敏感性与普通小球藻相当或略高。