Yi Xionghai, Ding Hui, Lu Yitong, Liu Haohua, Zhang Min, Jiang Wei
Department of Resource and Environment Science, Shanghai Jiao Tong University, 2678 Qi Xin Road, Shanghai 201101, People's Republic of China.
Chemosphere. 2007 Jul;68(8):1576-81. doi: 10.1016/j.chemosphere.2007.02.035. Epub 2007 Apr 12.
Alachlor has been widely used in agriculture all over the world. It is suggested that it may be a carcinogen and also an environmental estrogen. In this paper, the physiological and biochemical perturbations of crucian carp (Carassius auratus) exposed to alachlor at different concentrations over 60 days were investigated. The gonadosomatic index (GSI) and hepatosomatic index (HSI) were measured. The activity of hepatic antioxidant defense and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the content of glutathione (GSH) were determined and compared with the control group. The result showed that GSI and HSI decreased significantly (P<0.05) in almost all treatments. The activities of SOD, CAT and GST were induced continuously (P<0.05), while the content of reduced glutathione (GSH) was inhibited on the whole. These changes reflect that the antioxidant systems of the tested fishes were affected. The possible defense mechanistic implications about the changes were thus discussed. Furthermore, hepatic SOD and GST were sensitive to alachlor at low concentration, indicating that they might be potential biomarkers in early detection of alachlor contamination in aquatic ecosystems.
甲草胺已在全球农业中广泛使用。据推测，它可能是一种致癌物，也是一种环境雌激素。本文研究了鲫鱼（Carassius auratus）在60天内暴露于不同浓度甲草胺下的生理生化扰动情况。测量了性腺指数（GSI）和肝体指数（HSI）。测定了肝脏抗氧化防御和解毒酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽S-转移酶（GST）的活性以及谷胱甘肽（GSH）的含量，并与对照组进行了比较。结果表明，几乎所有处理组的GSI和HSI均显著降低（P<0.05）。SOD、CAT和GST的活性持续诱导（P<0.05），而还原型谷胱甘肽（GSH）的含量总体上受到抑制。这些变化反映了受试鱼类的抗氧化系统受到了影响。因此讨论了这些变化可能的防御机制意义。此外，肝脏SOD和GST对低浓度甲草胺敏感，表明它们可能是水生生态系统中甲草胺污染早期检测的潜在生物标志物。
