Xu Su, Yang Jehoon, Shen Jun
Molecular Imaging Branch, National Institute of Mental Health, Bethesda, MD 20892-1527, USA.
Magn Reson Imaging. 2008 Apr;26(3):413-9. doi: 10.1016/j.mri.2007.07.010. Epub 2007 Dec 11.
The wide chemical shift dispersion and long T(1) of (13)C have allowed determination of in vivo magnetization transfer effects caused by aspartate aminotransferase and lactate dehydrogenase reactions using (13)C magnetic resonance spectroscopy. In this report, we demonstrate that these effects can be observed in the proton spectra by transferring the equilibrium magnetization of (13)C via the one-bond scalar coupling between (13)C and (1)H using an inverse insensitive nuclei enhanced by polarization transfer-based heteronuclear polarization transfer method. This inverse method allows a combination of the advantages of the long (13)C T(1) for maximum magnetization transfer and the high sensitivity of proton detection. The feasibility of this in vivo inverse polarization transfer approach was evaluated for detecting the (13)C magnetization transfer effect of aspartate aminotransferase and lactate dehydrogenase reactions from a 72.5-microl voxel in the rat brain at 11.7 T.
碳-13（¹³C）具有较宽的化学位移分散度和较长的T(1)弛豫时间，这使得利用¹³C磁共振波谱法能够测定体内由天冬氨酸转氨酶和乳酸脱氢酶反应所引起的磁化传递效应。在本报告中，我们证明，通过使用基于极化转移的异核极化转移方法，即通过¹³C与¹H之间的一键标量耦合来转移¹³C的平衡磁化，这些效应能够在质子谱中被观察到。这种反向方法能够结合¹³C较长的T(1)弛豫时间以实现最大磁化传递的优势以及质子检测的高灵敏度。我们评估了这种体内反向极化转移方法在11.7 T磁场下从大鼠脑内一个72.5微升的体素中检测天冬氨酸转氨酶和乳酸脱氢酶反应的¹³C磁化传递效应的可行性。
