Golman Klaes, in 't Zandt René, Thaning Mikkel
Amersham Health R and D AB, GE Healthcare, Medeon, SE-205, 12 Malmö, Sweden.
Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11270-5. doi: 10.1073/pnas.0601319103. Epub 2006 Jul 12.
The endogenous substance pyruvate is of major importance to maintain energy homeostasis in the cells and provides a window to several important metabolic processes essential to cell survival. Cell viability is therefore reflected in the metabolism of pyruvate. NMR spectroscopy has until now been the only noninvasive method to gain insight into the fate of pyruvate in the body, but the low NMR sensitivity even at high field strength has only allowed information about steady-state conditions. The medically relevant information about the distribution, localization, and metabolic rate of the substance during the first minute after the injection has not been obtainable. Use of a hyperpolarization technique has enabled 10-15% polarization of (13)C(1) in up to a 0.3 M pyruvate solution. i.v. injection of the solution into rats and pigs allows imaging of the distribution of pyruvate and mapping of its major metabolites lactate and alanine within a time frame of approximately 10 s. Real-time molecular imaging with MRI has become a reality.
内源性物质丙酮酸对于维持细胞内的能量平衡至关重要，并且为细胞存活所必需的几个重要代谢过程提供了一个窗口。因此，细胞活力反映在丙酮酸的代谢中。到目前为止，核磁共振波谱法一直是了解体内丙酮酸去向的唯一非侵入性方法，但即使在高场强下，核磁共振的低灵敏度也只能提供有关稳态条件的信息。注射后第一分钟内该物质的分布、定位和代谢率等与医学相关的信息一直无法获得。超极化技术的应用已使高达0.3 M的丙酮酸溶液中的（13）C（1）极化达到10 - 15%。将该溶液静脉注射到大鼠和猪体内，可以在大约10秒的时间内对丙酮酸的分布进行成像，并绘制其主要代谢产物乳酸和丙氨酸的图谱。利用磁共振成像进行实时分子成像已成为现实。
