Dinesen B
Corporate Research, Novo Nordisk A/S, Bagsvaerd, Denmark.
Horm Res. 1991;36 Suppl 1:11-6. doi: 10.1159/000182181.
To construct an immunochemical assay of human growth hormone (hGH) is difficult due to the complicated nature of the hormone. Several forms coexist in the circulation with a 22,000 molecular weight form dominating. Related hormones with up to 93% homology are also present. hGH with its 191 amino acids presents more than 10 epitopes. Furthermore, the existence of two binding proteins adds to the complexity of the assay. Despite intensive efforts involving establishment of international standards, analytical consensus has not yet been reached. It is concluded that more specific immunoassays should be established.
由于人生长激素（hGH）性质复杂，构建其免疫化学测定法颇具难度。多种形式的hGH同时存在于血液循环中，其中分子量为22,000的形式占主导。还存在同源性高达93%的相关激素。含有191个氨基酸的hGH呈现出10多个表位。此外，两种结合蛋白的存在增加了测定的复杂性。尽管为建立国际标准付出了巨大努力，但尚未达成分析共识。结论是应建立更具特异性的免疫测定法。
