Hostacká A, Ciznár I
Slovenská zdravotnícka univerzita, Bratislava, Slovenská republika.
Epidemiol Mikrobiol Imunol. 2007 Nov;56(4):186-90.
To evaluate the effect of six culture media (five complex and one mineral) on biofilm formation and response to oxidative stress in Pseudomonas aeruginosa (3 strains) and Vibrio cholerae non-O1 (3 strains).
Biofilm formation was quantitatively determined by a crystal violet absorption assay. The bacterial response to oxidative stress evoked by hydrogen peroxide was visualized as a zone of clearing around the disc after 24 h incubation at 37 degrees C.
For both of the bacterial species studied, biofilm formation was the highest after cultivation in tryptone soya broth (TSM) or in TSM supplemented with 8% glucose (TSM+GL), being the lowest in mineral medium (MM). V. cholerae non O1 strains were 1.4 to 4.3 times more responsive on average to oxidative stress depending on culture medium as compared with P. aeruginosa strains. The culture medium had no significant effect on H2O2 evoked by response to oxidative stress in vibrios in contrast to P. aeruginosa. In P. aeruginosa, the highest mean resistance to H2O2 was observed after cultivation in peptone water while the most sensitive cells were found after incubation in TSM+GL and MM.
The culture medium composition influnced biofilm formation in both of the bacterial species tested and had a considerable effect on response to oxidative stress in P. aeruginosa.
评估六种培养基（五种复合培养基和一种矿物培养基）对铜绿假单胞菌（3株）和非O1群霍乱弧菌（3株）生物膜形成及氧化应激反应的影响。
通过结晶紫吸收试验定量测定生物膜形成。在37℃孵育24小时后，将细菌对过氧化氢引发的氧化应激反应可视化为滤纸片周围的清亮区域。
对于所研究的两种细菌，在胰蛋白胨大豆肉汤（TSM）或添加8%葡萄糖的TSM（TSM+GL）中培养后生物膜形成最高，在矿物培养基（MM）中最低。与铜绿假单胞菌菌株相比，非O1群霍乱弧菌菌株对氧化应激的平均反应性根据培养基不同为其1.4至4.3倍。与铜绿假单胞菌不同，培养基对霍乱弧菌氧化应激反应引发的H2O2无显著影响。在铜绿假单胞菌中，在蛋白胨水中培养后观察到对H2O2的平均抗性最高，而在TSM+GL和MM中孵育后发现最敏感的细胞。
培养基成分影响了所测试的两种细菌的生物膜形成，并对铜绿假单胞菌的氧化应激反应有相当大的影响。
