Slovak Medical University, 833 03, Bratislava, Slovak Republic.
Folia Microbiol (Praha). 2010 Jan;55(1):75-8. doi: 10.1007/s12223-010-0012-y. Epub 2010 Mar 25.
The effect of different cultivation temperatures (30 and 37 degrees C) and pH of the media (5.5, 7.5, 8.5) on the biofilm production was compared in Pseudomonas aeruginosa, Klebsiella pneumoniae, and Vibrio cholerae non-O1 and O1 using the crystal-violet test for estimation of quantitative production of the biofilm. Decrease (46.4-98.4 %) in the biofilm production was observed at 37 degrees C in 8 of the tested strains (P. aeruginosa three strains, K pneumoniae two, V. cholerae non-O1 two, and V. cholerae O1 one strain) compared with the production at 30 degrees C. On the other hand, five strains (P. aeruginosa 1, K. pneumoniae 3, V. cholerae non-O1 1) exhibited under these conditions a higher biofilm production (103-143 %). However, this difference was not significant (p = 0.196). Increased pH lead to a higher biofilm production using all media tested. In P. aeruginosa the biofilm production at pH 8.5 was 139-244 %, at pH 7.5 136-164 % in comparison with pH 5.5. Similarly, in K. pneumoniae the biofilm production increased to 151-319 % at pH 8.5 while with the drop of pH to 7.5 the biofilm production was 113-177 % compared with pH 5.5. In V. cholerae non-O1 and O1 the biofilm production reached 204-329 % at pH 8.5, and 123-316 % at pH 7.5 (compared with the production at pH 5.5). An increase in biofilm production represented an average of 169 % (p = 0.001) at pH change from 5.5 to 7.5, with the rise of pH from 5.5 to 8.5 caused an average difference of 229 % (p = 0.001).
我们比较了不同培养温度（30 和 37°C）和培养基 pH 值（5.5、7.5、8.5）对铜绿假单胞菌、肺炎克雷伯菌和非 O1 霍乱弧菌和 O1 霍乱弧菌生物膜产生的影响，使用结晶紫试验估计生物膜的定量产生。与 30°C 相比，在 8 株测试菌株（铜绿假单胞菌 3 株、肺炎克雷伯菌 2 株、非 O1 霍乱弧菌 2 株和 O1 霍乱弧菌 1 株）中，37°C 时生物膜产生减少了 46.4-98.4%。另一方面，在这些条件下，有 5 株菌株（铜绿假单胞菌 1 株、肺炎克雷伯菌 3 株、非 O1 霍乱弧菌 1 株）表现出更高的生物膜产生（103-143%）。然而，这种差异并不显著（p=0.196）。所有测试培养基的 pH 值升高都会导致生物膜产生增加。在铜绿假单胞菌中，pH 值为 8.5 时的生物膜产生量为 pH 值为 5.5 时的 139-244%，pH 值为 7.5 时为 136-164%。同样，在肺炎克雷伯菌中，pH 值升高到 8.5 时生物膜产生增加到 151-319%，而 pH 值降低到 7.5 时生物膜产生为 pH 值为 5.5 时的 113-177%。非 O1 和 O1 霍乱弧菌的生物膜产生量分别达到 pH 值为 8.5 时的 204-329%和 pH 值为 7.5 时的 123-316%（与 pH 值为 5.5 时的产生量相比）。pH 值从 5.5 升高到 7.5 时，生物膜产生增加了 169%（p=0.001），而 pH 值从 5.5 升高到 8.5 时，平均差异为 229%（p=0.001）。
