Kalicińska Agnieszka, Tyski Stefan
Zakład Mikrobiologii Farmaceutycznej Warszawski Uniwersytet Medyczny.
Med Dosw Mikrobiol. 2009;61(3):243-52.
The aim of this study was to analyse P aeruginosa biofilm growing in three media, using three methods of biofilm staining: with 2% crystal violet solution (CV), with 1% 2,3,5-triphenyltetrazolium chloride solution (TTC) and 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium bromide (MTT). Using the method with MTT solution we could observe growth of strains forming biofilm both slowly or quickly. Achieved values of absorbation allow to observe dynamic of growing selected strains in the period of time. Among three groups of analysed strains, the biggest percentage of medium growing strains were achieved, while in the fourth group (strains isolated from spit of patients with cystic fibrosis) the biggest percentage of low growing strains was observed. Achieved results allow to select the strains quickly forming biofilm, the best media (LB, TSB2%glu) and method of biofilm staining (MTT), which will be used in the further studies.
本研究的目的是使用三种生物膜染色方法，分析在三种培养基中生长的铜绿假单胞菌生物膜：用2%结晶紫溶液（CV）、1% 2,3,5-三苯基氯化四氮唑溶液（TTC）和3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑溴盐（MTT）。使用MTT溶液的方法，我们可以观察到形成生物膜的菌株生长缓慢或快速。获得的吸光度值有助于观察所选菌株在一段时间内的生长动态。在三组分析菌株中，生长于培养基中的菌株比例最高，而在第四组（从囊性纤维化患者痰液中分离的菌株）中，生长缓慢的菌株比例最高。所取得的结果有助于快速选择形成生物膜的菌株、最佳培养基（LB、TSB2%葡萄糖）和生物膜染色方法（MTT），这些将用于进一步的研究。
