Moco Sofia, Capanoglu Esra, Tikunov Yury, Bino Raoul J, Boyacioglu Dilek, Hall Robert D, Vervoort Jacques, De Vos Ric C H
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands.
J Exp Bot. 2007;58(15-16):4131-46. doi: 10.1093/jxb/erm271. Epub 2007 Dec 7.
Fruit maturation and tissue differentiation are important topics in plant physiology. These biological phenomena are accompanied by specific alterations in the biological system, such as differences in the type and concentration of metabolites. The secondary metabolism of tomato (Solanum lycopersicum) fruit was monitored by using liquid chromatography (LC) coupled to photo-diode array (PDA) detection, fluorescence detection (FD), and mass spectrometry (MS). Through this integrated approach different classes of compounds were analysed: carotenoids, xanthophylls, chlorophylls, tocopherols, ascorbic acid, flavonoids, phenolic acids, glycoalkaloids, saponins, and other glycosylated derivatives. Related metabolite profiles of peel and flesh were found between several commercial tomato cultivars indicating similar metabolite trends despite the genetic background. For a single tomato cultivar, metabolite profiles of different fruit tissues (vascular attachment region, columella and placenta, epidermis, pericarp, and jelly parenchyma) were examined at the green, breaker, turning, pink, and red stages of fruit development. Unrelated to the chemical nature of the metabolites, behavioural patterns could be assigned to specific ripening stages or tissues. These findings suggest spatio-temporal specificity in the accumulation of endogenous metabolites from tomato fruit.
果实成熟和组织分化是植物生理学中的重要课题。这些生物学现象伴随着生物系统的特定变化，例如代谢物类型和浓度的差异。通过使用液相色谱（LC）与光电二极管阵列（PDA）检测、荧光检测（FD）和质谱（MS）联用的方法，对番茄（Solanum lycopersicum）果实的次生代谢进行了监测。通过这种综合方法，分析了不同类别的化合物：类胡萝卜素、叶黄素、叶绿素、生育酚、抗坏血酸、黄酮类化合物、酚酸、糖苷生物碱、皂苷和其他糖基化衍生物。在几个商业番茄品种之间发现了果皮和果肉的相关代谢物谱，表明尽管遗传背景不同，但代谢物趋势相似。对于单个番茄品种，在果实发育的绿色、破色、转色、粉红和红色阶段，检查了不同果实组织（维管束附着区域、果心和胎座、表皮、果皮和果冻薄壁组织）的代谢物谱。与代谢物的化学性质无关，可以将行为模式归因于特定的成熟阶段或组织。这些发现表明番茄果实内源性代谢物积累具有时空特异性。
