Arruda Henrique Silvano, Angolini Célio Fernando Figueiredo, Eberlin Marcos Nogueira, Pastore Glaucia Maria, Marostica Junior Mario Roberto
Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, Rua Monteiro Lobato 80, Campinas 13083-862, São Paulo, Brazil.
Institute of Chemistry, University of Campinas, Rua Josué de Castro s/n, Campinas 13083-970, São Paulo, Brazil.
Foods. 2023 Sep 16;12(18):3456. doi: 10.3390/foods12183456.
Araticum is a native species of the Brazilian Cerrado with a high potential for exploitation. Several studies have stated that araticum is a rich source of phytochemicals with multifaceted biological actions. However, little information is available regarding the characterization of phytochemicals found in the pulp of this fruit. In this context, this study aimed to carry out a comprehensive characterization of phytochemicals present in the araticum pulp using ultra-high-performance liquid chromatography coupled to a quadrupole time-of-flight mass spectrometer (UHPLC-ESI-QTOF-MS/MS). The antioxidant potential of araticum pulp was also evaluated. UHPLC-ESI-QTOF-MS/MS profiling of the phytochemicals allowed for the identification and annotation of 139 phytochemicals, including organic acids, jasmonates, iridoids, phenolic compounds, alkaloids, annonaceous acetogenins, fatty acid derivatives, and other compounds. Among them, 116 compounds have been found for the first time in araticum pulp. Phenolic compounds and their derivatives represented about 59% of the phytochemicals identified in the extract. Moreover, araticum pulp showed high total phenolic compound content and antioxidant activity. The majority of identified phytochemicals have been associated with key roles in the plant's defense mechanisms against biotic and abiotic stress factors in the Cerrado environment. Furthermore, many of these phytochemicals found in the araticum pulp are already widely recognized for their beneficial effects on human health. Our findings showed that the araticum fruit contains different classes of phytochemicals that exert various biological activities, both in the plant itself and in humans.
刺果番荔枝是巴西塞拉多的本土物种，具有很高的开发潜力。多项研究表明，刺果番荔枝是一种富含植物化学物质的来源，具有多方面的生物活性。然而，关于这种水果果肉中植物化学物质的特征信息却很少。在此背景下，本研究旨在利用超高效液相色谱与四极杆飞行时间质谱联用技术（UHPLC-ESI-QTOF-MS/MS）对刺果番荔枝果肉中的植物化学物质进行全面表征。同时还评估了刺果番荔枝果肉的抗氧化潜力。通过对植物化学物质的UHPLC-ESI-QTOF-MS/MS分析，鉴定并注释了139种植物化学物质，包括有机酸、茉莉酸酯、环烯醚萜、酚类化合物、生物碱、番荔枝内酯、脂肪酸衍生物和其他化合物。其中，有116种化合物是首次在刺果番荔枝果肉中发现。酚类化合物及其衍生物约占提取物中鉴定出的植物化学物质的59%。此外，刺果番荔枝果肉显示出较高的总酚化合物含量和抗氧化活性。大多数鉴定出的植物化学物质在植物抵御塞拉多环境中生物和非生物胁迫因素的防御机制中发挥着关键作用。此外，在刺果番荔枝果肉中发现的许多这些植物化学物质因其对人类健康的有益作用而早已广为人知。我们的研究结果表明，刺果番荔枝果实含有不同种类的植物化学物质，这些物质在植物本身和人类中都发挥着各种生物活性。
