Zhang Xiao-yu, Tao Jun-yan, Zhao Lei, Huang Zhi-jun, Xiong Fu-liang, Zhang Shu-ling, Li Chong-ming, Xiao Fei
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Chin Med J (Engl). 2007 Nov 20;120(22):1992-8.
Melilotus suaveolens Ledeb (M. suaveolens Ledeb) has long been used as a folk medicine in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant.
Petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, aqueous fraction were obtained from ethanol extract of M. suaveolens Ledeb and evaluated by high performance liquid chromatography (HPLC). While dexamethasone (DM) was used as a positive control, the effects of different solution fractions of ethanol extract on tumor necrosis factor alpha (TNF-alpha) mRNA, cyclooxygenase 2 (COX-2) mRNA, COX-2 and nuclear factor kappaB (NF-kappaB) of LPS-stimulated RAW 264.7 cells were studied by real-time PCR, Western blot analysis and immunocytochemical assay, respectively.
Coumarin was one of the main ingredients in different solution fractions of ethanol extract except the aqueous fraction with no inflammatory effect. The petroleum ether fraction, ethyl acetate fraction and n-butanol fraction of ethanol extract could inhibit the production of TNF-alpha mRNA, COX-2 mRNA and NF-kappaB to some extent.
Different solution fractions of ethanol extract from M. suaveolens Ledeb had similar anti-inflammatory effect as did dexamethasone except the aqueous fraction. Coumarin was likely to be essential to the anti-inflammatory effect, and other ingredients might attribute to their different anti-inflammatory effects from the HPLC fingerprint.
草木犀长期以来一直被用作炎症相关治疗的民间药物。本研究旨在确定该植物的抗炎作用。
从草木犀乙醇提取物中获得石油醚部位、乙酸乙酯部位、正丁醇部位、水部位，并通过高效液相色谱（HPLC）进行评估。以地塞米松（DM）作为阳性对照，分别采用实时荧光定量PCR、蛋白质免疫印迹分析和免疫细胞化学分析法研究乙醇提取物不同溶液部位对脂多糖（LPS）刺激的RAW 264.7细胞中肿瘤坏死因子α（TNF-α）mRNA、环氧化酶2（COX-2）mRNA、COX-2和核因子κB（NF-κB）的影响。
香豆素是乙醇提取物不同溶液部位中的主要成分之一，除水部位无抗炎作用外。乙醇提取物的石油醚部位、乙酸乙酯部位和正丁醇部位在一定程度上可抑制TNF-α mRNA、COX-2 mRNA和NF-κB的产生。
草木犀乙醇提取物的不同溶液部位除水部位外，具有与地塞米松相似的抗炎作用。香豆素可能是抗炎作用所必需的，其他成分可能因其不同的抗炎作用而在HPLC指纹图谱中有所体现。
