Department of Hepatology & Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430072, Department of Neurosurgery and Central Lab, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, P.R. China.
Evid Based Complement Alternat Med. 2010 Mar;7(1):97-106. doi: 10.1093/ecam/nem165. Epub 2007 Nov 14.
Melilotus suaveolens Ledeb is a traditional medicinal plant for treating inflammation-related disease. This explores the inner anti-inflammatory mechanism of n-butanol extract from M. suaveolens Ledeb. Inflammatory cellular model was established by lipopolysaccharide intervention on RAW264.7 cell line. Levels of secreted cytokines TNF-α, IL-1β, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-α, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-κB and ingredients in the extract were assayed by ELISA, real time quantitative PCR, western blot, immunocytochemical test and HPLC fingerprint test, respectively. As a result, the extract could not only markedly reduce the production of pro-inflammatory mediators to different extents by blocking NF-κB activation but also promote the release of anti-inflammatory mediator HO-1 significantly. Each 1 g extract contained 0.023531 mg coumarin and another two high polar ingredients, probably saponins. It can be concluded that the extract has similar effects on antagonizing pro-inflammatory mediators and cytokines like Dexamethasone, and has effects on promoting the production of anti-inflammatory mediators.
草木樨状黄耆是一种传统的药用植物，用于治疗与炎症相关的疾病。本研究探索了草木樨状黄耆正丁醇提取物的内在抗炎机制。通过脂多糖干预 RAW264.7 细胞系建立炎症细胞模型。通过 ELISA、实时定量 PCR、western blot、免疫细胞化学试验和 HPLC 指纹图谱试验分别测定上清液中 TNF-α、IL-1β、IL-6、NO 和 IL-10 等细胞因子的分泌水平、TNF-α、COX-2、iNOS 和 HO-1 的 mRNA 表达、COX-2 和 HO-1 的蛋白表达、NF-κB 的激活以及提取物中的成分。结果表明，该提取物通过阻断 NF-κB 激活，不仅能显著降低促炎介质的产生，而且能显著促进抗炎介质 HO-1 的释放。每 1 g 提取物中含有 0.023531 mg 香豆素和另外两种高极性成分，可能是皂苷。可以得出结论，该提取物具有与地塞米松类似的拮抗促炎介质和细胞因子的作用，并具有促进抗炎介质产生的作用。
