Duarte Fernanda Oliveira, Sene-Fiorese Marcela, Manzoni Marla Simone Jovenasso, de Freitas Laura Franco, Cheik Nadia Carla, Garcia de Oliveira Duarte Ana Claudia, Nonaka Keico Okino, Dâmaso Ana
Departamento de Educação Física e Motricidade Humana, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Paulo, Brazil.
Nutrition. 2008 Feb;24(2):177-86. doi: 10.1016/j.nut.2007.10.012.
We investigated the effects of severe caloric restriction and refeeding with a high-fat diet on lipid uptake by visceral adipose fat and lipid profile in rats.
Rats were assigned to six groups: a chow diet (C), a high-fat diet (H), severe caloric restriction (SC and SH), and severe caloric restriction plus refeeding (SC-r and SH-r) during 8 wk. All animals were killed by decapitation 4 h after intragastric administration of [1-14C] triolein ( approximately 0.5 g, 0.3 muCi/rat). Liver; visceral retroperitoneal (RET), epididymal (EPI), and omental (VIS) white adipose tissues; brown adipose tissue; and intestine were immediately removed and weighed. The whole intestine was withdrawn and homogenized to determine lipid uptake. Total cholesterol, high-density lipoprotein (HDL), and triacylglycerol in plasma were determined enzymatically.
The SC and SH groups showed reduced visceral adiposity, although this effect was more evident in the SC group. The SC group had greater lipid absorption in the VIS than the C group. The SH treatment increased RET and VIS lipid uptake in relation to the H group. The SH-r treatment increased RET and VIS adiposity. HDL cholesterol decreased with caloric restriction in the SC and SH groups. The SH-r treatment did not increase HDL cholesterol.
Severe caloric restriction decreased visceral adiposity even in rats in the H group but did not reduce the risk of development of dyslipidemia. Therefore, food restriction plus refeeding with a high-fat diet increase the risk of development of visceral adiposity and dyslipidemia.
我们研究了严重热量限制及高脂饮食再喂养对大鼠内脏脂肪脂质摄取及血脂谱的影响。
将大鼠分为六组：正常饮食组（C）、高脂饮食组（H）、严重热量限制组（SC和SH）以及严重热量限制加再喂养组（SC-r和SH-r），为期8周。在胃内给予[1-14C]三油酸甘油酯（约0.5 g，0.3 μCi/只大鼠）4小时后，所有动物断头处死。立即取出肝脏、内脏腹膜后（RET）、附睾（EPI）和网膜（VIS）白色脂肪组织、棕色脂肪组织及肠道并称重。取出整个肠道并匀浆以测定脂质摄取。采用酶法测定血浆总胆固醇、高密度脂蛋白（HDL）和三酰甘油。
SC组和SH组内脏脂肪增多情况减少，尽管这种效应在SC组更明显。与C组相比，SC组VIS的脂质吸收更多。与H组相比，SH处理增加了RET和VIS的脂质摄取。SH-r处理增加了RET和VIS的脂肪量。SC组和SH组中，热量限制使HDL胆固醇降低。SH-r处理未增加HDL胆固醇。
即使是H组大鼠中，严重热量限制也会降低内脏脂肪量，但并未降低血脂异常发生的风险。因此，食物限制加高脂饮食再喂养会增加内脏脂肪增多和血脂异常发生的风险。
