Salman B, Kerem M, Bedirli A, Katircioglu H, Ofluoglu E, Akin O, Onbasilar I, Ozsoy S, Haziroglu R
Department of General Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey.
Colorectal Dis. 2008 Jun;10(5):469-78. doi: 10.1111/j.1463-1318.2007.01426.x. Epub 2007 Dec 5.
Algae, which are used as supplementary nutrients in various countries, are products rich in protein, vitamins and minerals. The aim of this study was to investigate the effects of algae extracts on the healing of colonic anastomosis in malnourished rats.
Seventy-two rats were randomized to three groups. Group 1 was fed with standard diet for 15 days, before and after the colonic anastomosis. Groups 2 and 3 were fed with a malnutrition diet for 15 days prior to colonic anastomosis and then with the basic diet for 15 days there after. Group 3 also received an extract of algae derived from Cholerella sp. via oral gavage postoperatively, in addition to the basic diet. Rats were killed on the 3rd, 7th and 15th postoperative day. Blood samples were collected to evaluate prealbumin, transferring and albumin levels. Anastomotic bursting pressures (BPs), histopathology and tissue hydroxyproline levels were evaluated after killing.
In group 3, the prealbumin level on the 3rd postoperative day and transferrin and albumin levels on the 7th and 15th postoperative days were significantly increased compared with the other groups (P < 0.05). Tissue hydroxyproline levels and anastomotic BPs of group 3 were significantly higher than in group 2 on the 3rd, 7th and 15th postoperative days (P < 0.05). Histopathological examination of the anastomosis revealed significantly better healing patterns for group 3 than for groups 1 and 2 (P < 0.05).
Extract derived from Cholerella sp. microalgae has favourable effects on healing of experimental colon anastomoses.
藻类在各国被用作补充营养物质，是富含蛋白质、维生素和矿物质的产品。本研究旨在探讨藻类提取物对营养不良大鼠结肠吻合口愈合的影响。
将72只大鼠随机分为三组。第1组在结肠吻合术前和术后均喂食标准饮食15天。第2组和第3组在结肠吻合术前喂食营养不良饮食15天，之后喂食基础饮食15天。第3组除基础饮食外，术后还通过灌胃给予小球藻属藻类提取物。在术后第3、7和15天处死大鼠。采集血样以评估前白蛋白、转铁蛋白和白蛋白水平。处死大鼠后评估吻合口破裂压力（BP）、组织病理学和组织羟脯氨酸水平。
与其他组相比，第3组术后第3天的前白蛋白水平以及术后第7天和第15天的转铁蛋白和白蛋白水平显著升高（P < 0.05）。术后第3、7和15天，第3组的组织羟脯氨酸水平和吻合口BP显著高于第2组（P < 0.05）。吻合口的组织病理学检查显示，第3组的愈合模式明显优于第1组和第2组（P < 0.05）。
小球藻属微藻提取物对实验性结肠吻合口愈合具有良好作用。
