State Key Laboratory of Animal Nutrition, China Agricultural University, No 2 Yuanmingyuan West Road, Beijing 100193, China.
J Ethnopharmacol. 2010 Mar 24;128(2):412-8. doi: 10.1016/j.jep.2010.01.035. Epub 2010 Jan 18.
Soybeans are known to stimulate food allergies; however, current therapies are lacking in alleviating hypersensitivity. The present study investigated whether Forsythia suspensa extract could attenuate purified soybean beta-conglycinin-induced anaphylactic reactions in weaned piglets.
Eighteen 14-day-old piglets were sensitized and boosted by oral administration of purified beta-conglycinin. Forsythia suspensa extract was supplemented in the diet after the initial sensitization and continued for the remainder of the experiment. Piglets were challenged on day 28, and anaphylactic symptoms, passive cutaneous anaphylaxis, plasma histamine and intestinal microbial flora were analyzed. T-cell proliferative responses and cytokine production were also determined.
Forsythia suspensa extract alleviated beta-conglycinin-induced anaphylactic symptoms by markedly reducing anaphylactic antibodies, mast cell degranulation, and histamine release while improving intestinal microbial flora. Furthermore, Forsythia suspensa extract significantly suppressed beta-conglycinin-induced T lymphocyte proliferation and IL-4 synthesis.
Forsythia suspensa extract protected beta-conglycinin-sensitized piglets from anaphylactic reactions. Forsythia suspensa extract may provide a novel effective therapy for soybean allergy.
大豆已知可引发食物过敏；然而，目前的治疗方法在缓解过敏反应方面有所欠缺。本研究旨在探讨连翘提取物是否可以减轻断奶仔猪对纯化大豆β-伴大豆球蛋白诱导的过敏反应。
18 头 14 日龄仔猪通过口服纯化的β-伴大豆球蛋白进行致敏和加强。在初始致敏后，饲料中添加连翘提取物，并在实验的剩余时间内继续添加。第 28 天对仔猪进行挑战，并分析过敏症状、被动皮肤过敏反应、血浆组胺和肠道微生物菌群。还测定了 T 细胞增殖反应和细胞因子产生。
连翘提取物通过显著减少过敏抗体、肥大细胞脱颗粒和组胺释放，同时改善肠道微生物菌群，减轻了β-伴大豆球蛋白诱导的过敏症状。此外，连翘提取物显著抑制了β-伴大豆球蛋白诱导的 T 淋巴细胞增殖和 IL-4 合成。
连翘提取物可保护β-伴大豆球蛋白致敏的仔猪免受过敏反应。连翘提取物可能为大豆过敏提供一种新的有效治疗方法。
