Trávnicek M, Deptula W, Gazdic J
Institút výchovy a vzdelávania veterinárnych lekárov, Kosice.
Vet Med (Praha). 1991 Sep;36(9):561-7.
46 head of pregnant sheep, the Tsigaya breed, were subjected to the skin allergy test and subsequently divided into two groups. Sheep were in the 3rd month of gravidity and were a part of a flock consisting of 300 head, in which chlamydia-induced abortion was recorded in sheep. The skin allergic test was done by the Rodolakis et al. (1977) method, modified by us, to indicate the level of cell-mediated immune response. Simultaneously with it, serological examinations (complement fixation test--CFT) were performed to find out the levels of antibody against Ch. psittaci. The results of skin allergy test (SAT) and serological examination in sheep after bivalent vaccine administration are given in Tab. II. Of the total number of sheep ranked to vaccinated group, 18 head responded positively on SAT. After vaccination, 12 head responded positively though previously responded negatively. In vaccinated group one abort recorded in the sheep. No. 12 which was on the 0 day slightly positive in the skin test. High levels of antibody were found after abortion and the skin test was highly positive. The results of SAT and serological examination in sheep, when placebo was administered, are given in Tab. III. 6 sheep aborted in the group, placebo was administered, are given in Tab. III. 6 sheep aborted in the group, of which 5 were negative and one was slightly positive on the day 0 in SAT. In 4 sheep abort was accompanied with significant increase in humoral antibody against Ch. psittaci. In sheep which aborted and were negative in SAT on the day 0, a marked positivity has been indicated in the replicated SAT test.(ABSTRACT TRUNCATED AT 250 WORDS)
46头齐盖亚品种的怀孕母羊接受了皮肤过敏试验,随后被分为两组。这些母羊处于妊娠第3个月,是一个由300头羊组成的羊群的一部分,该羊群中曾有羊发生衣原体诱导的流产。皮肤过敏试验采用我们改良的罗多拉基斯等人(1977年)的方法,以指示细胞介导的免疫反应水平。与此同时,进行了血清学检查(补体结合试验——CFT),以确定抗鹦鹉热衣原体抗体的水平。二价疫苗接种后绵羊的皮肤过敏试验(SAT)和血清学检查结果见表二。在归入接种组的绵羊总数中,18头SAT呈阳性反应。接种疫苗后,12头之前反应为阴性的羊呈阳性反应。接种组有1头羊发生流产,即第12号羊,其在皮肤试验第0天呈弱阳性。流产后发现抗体水平很高,皮肤试验呈强阳性。给予安慰剂时绵羊的SAT和血清学检查结果见表三。给予安慰剂的组中有6头羊流产,其中5头SAT为阴性,1头在第0天呈弱阳性。在4头流产羊中,抗鹦鹉热衣原体的体液抗体显著增加。在流产且第0天SAT为阴性的羊中,重复的SAT试验显示明显阳性。(摘要截于250字)