对背景哺乳动物溶酶体β-半乳糖苷酶的差异抑制提高了LacZ标记白血病细胞的检测灵敏度。
Differential suppression of background mammalian lysosomal beta-galactosidase increases the detection sensitivity of LacZ-marked leukemic cells.
作者信息
Hendrikx P J, Martens A C, Visser J W, Hagenbeek A
机构信息
Institute of Hematology, Erasmus University Rotterdam, The Netherlands.
出版信息
Anal Biochem. 1994 Nov 1;222(2):456-60. doi: 10.1006/abio.1994.1516.
A method is described for the detection of Escherichia coli beta-galactosidase-expressing leukemic cells in ex vivo bone marrow samples. 4-Methylumbelliferyl-beta-D-galactopyranoside is used as a substrate in a kinetic assay. D-Galactose is used to suppress endogenous lysosomal beta-galactosidase activity, yielding a sixfold increase in sensitivity. With this assay, the detection limit is one leukemic cell per 10(4) normal bone marrow cells.
描述了一种用于检测离体骨髓样本中表达大肠杆菌β-半乳糖苷酶的白血病细胞的方法。在动力学测定中使用4-甲基伞形酮基-β-D-吡喃半乳糖苷作为底物。用D-半乳糖抑制内源性溶酶体β-半乳糖苷酶活性，灵敏度提高了六倍。通过该测定，检测限为每10⁴个正常骨髓细胞中有一个白血病细胞。
Zotero 插件