van Verseveld H W, Metwally M, el Sayed M, Osman M, Schrickx J M, Stouthamer A H
Vrije Universiteit, Biological Laboratories, Department of Microbiology, Amsterdam, The Netherlands.
Antonie Van Leeuwenhoek. 1991 Oct-Nov;60(3-4):313-23. doi: 10.1007/BF00430372.
Aspergillus niger has been grown in glucose- and maltose-limited recycling cultures to determine the maximum growth yield, the maximum product yield for glucoamylase production, and the maintenance requirements at very slow specific growth rates. Using the linear equation for substrate utilization, and using the experimental data from both recycling experiments, both the maximum growth yield, Yxsm, and the maximum product yield, Ypsm, could be determined. The values estimated were 157 g biomass per mol maltose for Yxsm and 100 g protein per mol maltose for Ypsm. Expressed on a C1-basis these values are 0.52 and 0.36 C-mole per C-mol for respectively Yxsm and Ypsm. The found value for Ypsm is half the value found for alkaline serine protease production in Bacillus licheniformis, and it can be concluded that formation of extracellular protein is more energy consuming in filamentous fungi than in prokaryotic organisms. Maintenance requirements are no significant factor during growth of Aspergillus niger, and reported maintenance requirements are most probably due to differentiation.
黑曲霉已在葡萄糖和麦芽糖限制的循环培养物中生长，以确定最大生长产量、糖化酶生产的最大产物产量以及极低比生长速率下的维持需求。利用底物利用的线性方程，并结合两个循环实验的实验数据，可以确定最大生长产量Yxsm和最大产物产量Ypsm。估计得到的Yxsm值为每摩尔麦芽糖产生157克生物量，Ypsm值为每摩尔麦芽糖产生100克蛋白质。以C1为基础表示，Yxsm和Ypsm的值分别为每C摩尔0.52和0.36 C摩尔。所发现的Ypsm值是地衣芽孢杆菌中碱性丝氨酸蛋白酶生产所发现值的一半，可以得出结论，丝状真菌中细胞外蛋白质的形成比原核生物消耗更多能量。维持需求在黑曲霉生长过程中不是显著因素，报道的维持需求很可能是由于分化所致。
