Meggs William J, Brewer Kori L
Department of Emergency Medicine, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA.
J Med Toxicol. 2007 Sep;3(3):89-93. doi: 10.1007/BF03160916.
This work exposed rats to low levels of the organophosphate insecticide chlorpyrifos and monitored for toxic effects, including weight gain.
Rats received either a subcutaneous injection of chlorpyrifos, 5 mg/kg/day, or an equal volume of vehicle daily for 4 months. Subjects were observed for 30 minutes after injection for signs of acute toxicity. Body weights were recorded at baseline, 2 months, 3 months, and 4 months. At the end of the experiment, the weights of hearts, medial lobe of the livers, peri-nephric fat pads, and gastrocnemius muscles were recorded. Effects of chlorpyrifos on adipocyte differentiation in culture were studied. Results were compared using RMANOVA.
No signs of acute cholinergic toxicity were observed after injections in any subject. Rats in the 5 mg/kg group were significantly heavier than those in the control group by 2 months (335.7 +/- 16.7 g vs. 318.6 +/- 15.8 g; p = 0.034). This difference increased at 3 months (350.1 +/- 16.4 g vs. 322.3 +/- 21.3 g p = 0.006) and 4 months (374.4 +/- 22.2 g vs. 340.2 +/- 25.2 g p = 0.006). At 4 months, the weights of the perinephric fat pads were significantly increased in the chlorpyrifos group relative to controls (2.867 + 0.516 vs. 1.130 + 0.171, p = 0.0039). The two groups showed no weight differences between hearts, livers, and gastrocnemius muscles. Chlorpyrifos did not affect adipocyte differentiation in tissue culture.
Chronic exposure to chlorpyrifos at 5 mg/kg/day caused an increase in rat body weight when compared to controls. This increase was in adipose tissue. Chlorpyrifos did not induce differentiation of adipocytes in culture.
本研究让大鼠接触低剂量的有机磷杀虫剂毒死蜱，并监测其毒性作用，包括体重增加情况。
大鼠每日皮下注射5毫克/千克的毒死蜱或等量的赋形剂，持续4个月。注射后观察30分钟，查看急性毒性迹象。在基线、2个月、3个月和4个月时记录体重。实验结束时，记录心脏、肝脏中叶、肾周脂肪垫和腓肠肌的重量。研究了毒死蜱对培养中脂肪细胞分化的影响。使用重复测量方差分析比较结果。
注射后，任何受试大鼠均未观察到急性胆碱能毒性迹象。5毫克/千克组的大鼠在2个月时比对照组显著更重（335.7±16.7克对318.6±15.8克；p = 0.034）。这种差异在3个月时增大（350.1±16.4克对322.3±21.3克，p = 0.006），在4个月时也增大（374.4±22.2克对340.2±25.2克，p = 0.006）。在4个月时，毒死蜱组的肾周脂肪垫重量相对于对照组显著增加（2.867 + 0.516对1.130 + 0.171，p = 0.0039）。两组在心脏、肝脏和腓肠肌的重量上没有差异。毒死蜱在组织培养中不影响脂肪细胞分化。
与对照组相比，每天以5毫克/千克的剂量长期接触毒死蜱会导致大鼠体重增加。这种增加发生在脂肪组织中。毒死蜱在培养中不诱导脂肪细胞分化。
