Ozlu Tevfik, Celik Ilknur, Oztuna Funda, Bulbul Yilmaz, Ozsu Savas
Department of Chest Diseases, Karadeniz Technical University Medical School, Trabzon, Turkey.
Respiration. 2008;75(3):339-44. doi: 10.1159/000112472. Epub 2007 Dec 10.
Bacterial adherence to buccal epithelial cells in chronic smokers has been reported to increase in vitro. However, it is unclear whether such adherence increases in hosts without epithelial changes linked to chronic smoking.
This was an in vivo investigation of whether or not there was a change in the Streptococcus pneumoniae adherence to buccal epithelial cells of rats exposed to different periods and degrees of environmental cigarette smoke.
Eighteen rats were divided into acute and chronic exposure groups, each containing 9 rats. Both groups were further divided into 3 subgroups each, which were exposed to low or high levels of cigarette smoke, or none at all. After the administration of smoke, S. pneumoniae (ATCC 49619) was inoculated into the buccal mucosa. One hour later, buccal epithelial cell samples were taken and the number of bacteria adhering to 40 buccal epithelial cells was counted. Pre- and postinoculation values and the groups administered smoke and the control groups were then compared.
A significant increase in gram-positive diplococci adherence was determined in all groups after S. pneumoniae inoculation. S. pneumoniae was observed to adhere to buccal epithelial cells in all the control group rats and in those in the groups exposed to cigarette smoke. Pneumococcal adherence in the chronic exposure subgroups increased approximately 4-fold with low smoke exposure (p < 0.004) and approximately 7-fold with high smoke exposure (p < 0.0005).
S. pneumoniae adherence was significantly high in rats chronically exposed to a high level of cigarette smoke, but not in the acute exposure groups.
据报道，在体外实验中，慢性吸烟者口腔上皮细胞的细菌黏附增加。然而，尚不清楚在没有与慢性吸烟相关的上皮变化的宿主中，这种黏附是否会增加。
本研究旨在对暴露于不同时长和程度的环境香烟烟雾中的大鼠，探究肺炎链球菌对其口腔上皮细胞的黏附是否发生变化。
将18只大鼠分为急性暴露组和慢性暴露组，每组9只。两组再各自分为3个亚组，分别暴露于低水平或高水平香烟烟雾，或不暴露于任何烟雾。给予烟雾处理后，将肺炎链球菌（ATCC 49619）接种于口腔黏膜。1小时后，采集口腔上皮细胞样本，计数黏附于40个口腔上皮细胞上的细菌数量。然后比较接种前后的值以及烟雾处理组和对照组。
接种肺炎链球菌后，所有组革兰氏阳性双球菌黏附均显著增加。在所有对照组大鼠以及暴露于香烟烟雾的组中，均观察到肺炎链球菌黏附于口腔上皮细胞。慢性暴露亚组中，低烟雾暴露时肺炎球菌黏附增加约4倍（p < 0.004），高烟雾暴露时增加约7倍（p < 0.0005）。
长期暴露于高浓度香烟烟雾的大鼠中，肺炎链球菌黏附显著增加，但急性暴露组未出现这种情况。
