Raman A S, Swinburne A J, Fedullo A J
Chest. 1983 Jan;83(1):23-7. doi: 10.1378/chest.83.1.23.
Adherence to mucosal surfaces is necessary for bacterial colonization. The in-vitro adherence to type 25 Streptococcus pneumoniae to buccal epithelial cells was studied in 15 smokers, 15 nonsmokers, and 21 exsmokers. Background adherence in smokers and nonsmokers was similar, but smokers had a markedly increased pneumococcal adherence compared to nonsmokers (12.3 +/- 6.9 vs 0.7 +/- 0.4). This increase was not related to subject age or duration of cigarette use. Pneumococcal adherence in some exsmokers remained elevated for up to three years after smoking cessation. Incubation of nonsmokers' cells with smoker's saliva resulted in increased pneumococcal adherence to the nonsmokers' cells (1.1 +/- 0.099 to 8.2 +/- 4.4), suggesting mediation of pneumococcal adherence by a noncellular constituent of smokers' saliva. The increased pneumococcal adherence in cigarette smokers may promote oropharyngeal colonization and contribute to the increased risk of respiratory infection in cigarette smokers.
细菌定殖需要附着于黏膜表面。对15名吸烟者、15名不吸烟者和21名已戒烟者研究了25型肺炎链球菌在体外对颊上皮细胞的附着情况。吸烟者和不吸烟者的背景附着情况相似，但与不吸烟者相比，吸烟者的肺炎链球菌附着显著增加（12.3±6.9对0.7±0.4）。这种增加与受试者年龄或吸烟时长无关。部分已戒烟者在戒烟后长达三年的时间里，肺炎链球菌附着仍保持升高。用吸烟者的唾液孵育不吸烟者的细胞，导致肺炎链球菌对不吸烟者细胞的附着增加（从1.1±0.099增至8.2±4.4），提示吸烟者唾液中的一种非细胞成分介导了肺炎链球菌的附着。吸烟者肺炎链球菌附着增加可能促进口咽部定殖，并导致吸烟者呼吸道感染风险增加。
