Centre de recherche, Hôpital Laval, Institut universitaire de cardiologie et de pneumologie de l'Université Laval, Québec City, Quebec, Canada.
Inhal Toxicol. 2009 Nov;21(13):1148-53. doi: 10.3109/08958370902926692.
Cigarette smoke is associated with a high morbidity and mortality, and affects particularly the respiratory tract. Various in vitro models have been developed to study the effects of cigarette smoke on bronchial epithelial cells. To identify an adequate exposure model of cigarette smoke, we analysed the effects of cigarette smoke extract (CSE) and a smoking chamber on bronchial epithelial cells. The release of monocyte chemoattractant protein (MCP)-1, interleukin (IL)-10, and vascular endothelial growth factor (VEGF) was measured. Bronchial epithelial cells isolated from Sprague-Dawley rat (NRBE) were exposed to 3% CSE or air control every day for 3 days. In the second model, NRBE were placed in an air/liquid interface and exposed, in a smoking chamber, to whole smoke from 2 cigarettes, twice daily for 3 days. Levels of MCP-1, IL-10, and VEGF were measured by enzyme-linked immunosorbent assay (ELISA), 24 h after the last exposure. The pattern of MCP-1 production by bronchial epithelial cells was different between the two models. MCP-1 release was increased after 3 days of exposure in the CSE model, but was inhibited using the smoking chamber model. Production of IL-10 by NRBE was reduced after 3 days in both models. Finally, no difference was observed in the production of VEGF between the two models. CSE and the smoking chamber differently modulate bronchial epithelial cell mediator production, demonstrating that the model of cigarette smoke exposure used can influence the data obtained.
香烟烟雾与高发病率和死亡率有关，尤其会影响呼吸道。已经开发出各种体外模型来研究香烟烟雾对支气管上皮细胞的影响。为了确定适当的香烟烟雾暴露模型，我们分析了香烟烟雾提取物（CSE）和吸烟室对支气管上皮细胞的影响。测量单核细胞趋化蛋白-1（MCP-1）、白细胞介素（IL）-10 和血管内皮生长因子（VEGF）的释放。将来自 Sprague-Dawley 大鼠（NRBE）的支气管上皮细胞暴露于 3% CSE 或空气对照中，每天一次，持续 3 天。在第二个模型中，NRBE 被置于气/液界面，并在吸烟室内每天两次暴露于 2 支香烟的全烟雾中，持续 3 天。最后一次暴露后 24 小时，通过酶联免疫吸附测定（ELISA）测量 MCP-1、IL-10 和 VEGF 的水平。两种模型中支气管上皮细胞产生 MCP-1 的模式不同。CSE 模型中，暴露 3 天后 MCP-1 释放增加，但在吸烟室模型中被抑制。两种模型中，NRBE 产生的 IL-10 在 3 天后均减少。最后，两种模型之间 VEGF 的产生没有差异。CSE 和吸烟室以不同的方式调节支气管上皮细胞介质的产生，表明使用的香烟烟雾暴露模型会影响获得的数据。
