Zhang Chen, Gu Yan-Pin, Yuan Bao-Ping, Liu Yan, Guo Wei, Wei Hong-Chang, Lv Rong
Department of Pathology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Yao Cai. 2011 Sep;34(9):1399-402.
To investigate the effects of Leonurus japonicas on myocardial remodeling induced by isoproterenol (ISO) in rats.
The model of rat myocardial remodeling was established by subcutaneous injection of ISO (20,10 and 5 mg/kg for 3 days, subsequently 3 mg/kg for 7 days). Rats were randomly divided into five groups: Control, ISO, ISO + enalapril, ISO + Leonurus 8 g/ kg and ISO + Leonurus 16 g/kg. Recorded the values of LVSP, LVEDP, +/- dp/dt (max), cardiac output ( CO). Weighed the body weight (BW), heart weight (HW) and left ventricular weight (LVW) of rats to calculate the values of HW/BW and LVW/BW. The hydroxyproline contents were investigated by spectrophotometric measurement. The contents of type I collagen and type III collagen and ratio of I/III collagen were assessed by immunohistochemical stain.
Leonurus (16 g/kg x day(-1)) could increased the value of LVSP, + dp/dt(max), CO (P < 0.05). Leonurus (8 g/kg) could increased the value of -dp/dt(max), decreased the ratios of HW/BW and LVW/BW (P < 0.05), the contents of hydroxyproline, type I collagen, type III collagen and the ratios of I/III collagen (P < 0.05).
Leonurus at a dosage of 16 g/kg may improve the systolic function; Leonurus at a dosage of 8 g/kg may improve the diastolic function, down-regulate the expression of collagen and normalize the ratio of I/III collagen.
探讨益母草对异丙肾上腺素（ISO）诱导的大鼠心肌重塑的影响。
通过皮下注射ISO（20、10和5mg/kg，连续3天，随后3mg/kg，连续7天）建立大鼠心肌重塑模型。将大鼠随机分为五组：对照组、ISO组、ISO+依那普利组、ISO+8g/kg益母草组和ISO+16g/kg益母草组。记录左室收缩压（LVSP）、左室舒张末压（LVEDP）、±dp/dt（max）、心输出量（CO）。称取大鼠体重（BW）、心脏重量（HW）和左心室重量（LVW），计算HW/BW和LVW/BW值。采用分光光度法测定羟脯氨酸含量。通过免疫组织化学染色评估I型胶原和III型胶原含量及I/III型胶原比例。
益母草（16g/kg×d⁻¹）可提高LVSP、+dp/dt（max）、CO值（P<0.05）。益母草（8g/kg）可提高-dp/dt（max）值，降低HW/BW和LVW/BW比值（P<0.05），降低羟脯氨酸、I型胶原、III型胶原含量及I/III型胶原比例（P<0.05）。
16g/kg剂量的益母草可能改善收缩功能；8g/kg剂量的益母草可能改善舒张功能，下调胶原表达并使I/III型胶原比例正常化。
