[细胞凋亡在小鼠I型单纯疱疹病毒性心肌炎中的作用]
[The role of apoptosis in herpes simplex virus I type myocarditis of mouse].
作者信息
Hu Song-yan, Wang Xiao-dong, Tang Xing-shan
机构信息
Guangdong Food and Drug Vocational lollege, Guangzhou 510520, China.
出版信息
Zhong Yao Cai. 2007 Aug;30(8):989-91.
OBJECTIVE
To explore the relation among apoptosis of myocardial cell and the expression of TGF-beta1 in VMC.
METHODS AND RESULTS
In the experiment, Viral myocarditis (VMC) in BALB/C mouse was obtained by inculating herpes simplex virus I type into abdominal cavity. The rate of VMC was 91.67% after 3 - 35 days of virus infection. The apoptosis (78.33%) and expression of TGF-beta1 (81.76%) were found in the myocardium of infected mouse by TUNEL and immunohistochemical method and electron microscope. AI of myocardial cell was 34.14+/-11.56 - 26.14+/-10.2.
CONCLUSION
Apoptosis and expression of TGF-beta1 may participate in occurrence and development of VMC.
目的
探讨病毒性心肌炎（VMC）中心肌细胞凋亡与转化生长因子-β1（TGF-β1）表达之间的关系。
方法与结果
实验中，通过腹腔接种Ⅰ型单纯疱疹病毒建立BALB/C小鼠病毒性心肌炎模型。病毒感染3至35天后，VMC发生率为91.67%。采用TUNEL法、免疫组织化学法及电子显微镜观察发现，感染小鼠心肌组织存在细胞凋亡（78.33%）及TGF-β1表达（81.76%）。心肌细胞凋亡指数为34.14±11.56至26.14±10.2。
结论
细胞凋亡及TGF-β1表达可能参与了VMC的发生发展。
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