García-Rosado Esther, Cano Irene, Martín-Antonio Beatriz, Labella Alejandro, Manchado Manuel, Alonso M Carmen, Castro Dolores, Borrego Juan J
Department of Microbiology, University of Málaga, Málaga, Spain.
Int Microbiol. 2007 Sep;10(3):193-9.
Several microbial disease outbreaks in farm stocks of newly cultured sparid fish species, such as common seabream, redbanded seabream, and white seabream, were recorded from 2004 to 2006. This study describes the isolation and characterization of the potential causative agents, either bacteria or viruses, of these outbreaks. The isolated bacterial strains were characterized according to traditional taxonomical analyses and sequencing of a 16S rDNA fragment. Most bacteria were identified as Vibrio spp. and Photobacterium damselae subsp. damselae. The development of cytopathic effects (CPE) on different fish cell lines, the application of specific nested-PCR tests for infectious pancreatic necrosis virus (IPNV), viral nervous necrosis virus (VNNV) and viral hemorrhagic septicemia virus (VHSV), and subsequent sequence analyses were used for virus detection and identification. VNNV, related to the striped jack neural necrosis virus (SJNNV) genotype, and VHSV, related to the genotype Ia, were the only viruses detected. VNNV was isolated from the three fish species under study in five different outbreaks, whereas VHSV was isolated from common seabream and white seabream during two of these outbreaks. IPNV was not detected in any case.
2004年至2006年期间，记录了几种新养殖的鲷科鱼类（如黑鲷、红鳍笛鲷和白鲷）养殖种群中的几起微生物疾病暴发事件。本研究描述了这些暴发事件中潜在病原体（细菌或病毒）的分离和鉴定。根据传统分类分析和16S rDNA片段测序对分离出的细菌菌株进行了鉴定。大多数细菌被鉴定为弧菌属和美人鱼发光杆菌美人鱼亚种。通过观察不同鱼类细胞系上的细胞病变效应（CPE）、应用针对传染性胰腺坏死病毒（IPNV）、病毒性神经坏死病毒（VNNV）和病毒性出血性败血症病毒（VHSV）的特异性巢式PCR检测以及随后的序列分析来进行病毒检测和鉴定。检测到的唯一病毒是与条石鲷神经坏死病毒（SJNNV）基因型相关的VNNV和与Ia基因型相关的VHSV。在五次不同的暴发事件中，从所研究的三种鱼类中分离出了VNNV，而在其中两次暴发事件中，从黑鲷和白鲷中分离出了VHSV。在任何情况下均未检测到IPNV。
