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Synthesis of modified DNA by PCR with alkyne-bearing purines followed by a click reaction.

作者信息

Gramlich Philipp M E, Wirges Christian T, Gierlich Johannes, Carell Thomas

机构信息

Department of Chemistry, Ludwig-Maximilians University Munich, Butenandtstr. 13, D-81377 Munich, Germany.

出版信息

Org Lett. 2008 Jan 17;10(2):249-51. doi: 10.1021/ol7026015. Epub 2007 Dec 13.

DOI:10.1021/ol7026015
PMID:18076183
Abstract

Alkyne-bearing deazapurine triphosphates were prepared and successfully incorporated into DNA using the polymerase chain reaction (PCR). The obtained alkyne-labeled DNA was successfully used in a click reaction with galactose azide.

摘要

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