Gomez Dennis K, Baeck Gun Wook, Kim Ji Hyung, Choresca Casiano H, Park Se Chang
KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul 151-74, Republic of Korea.
J Invertebr Pathol. 2008 Mar;97(3):197-202. doi: 10.1016/j.jip.2007.10.012. Epub 2007 Nov 7.
One hundred eighteen samples (21 species) of wild marine invertebrates were collected from western and southern coastal area of Korean Peninsula. Four of 78 (18 species) samples collected at Namhae (South) area were positive for nodavirus in nested PCR test. Of the 40 samples (5 species) collected at Hwanghae (West) areas, all samples were negative for nodavirus in both RT-PCR and nested PCR tests. Positive nested PCR results were obtained from the following species: Charybdis bimaculata Charybdid crab; Pandalus hypsinotus Southern humpback shrimp and Mytilus galloprovincialis Mediterranean mussel. Phylogenetic analysis based on the partial nucleotide sequence (177 bases) of the RNA2 coat protein gene showed that the four strains were highly homologous (100%) and closely related to that of the known betanodaviruses, redspotted grouper nervous necrosis virus (RGNNV). These results indicate that nodavirus is present from wild marine invertebrates in the southern coastal areas of Korean Peninsula. These subclinically infected marine invertebrates may constitute an inoculum source for betanodavirus infection and cause mortality in cultured fishes in Korea.
从朝鲜半岛西部和南部沿海地区采集了118个野生海洋无脊椎动物样本（21种）。在南海（南部）地区采集的78个样本（18种）中，有4个在巢式PCR检测中诺达病毒呈阳性。在黄海（西部）地区采集的40个样本（5种）中，所有样本在RT-PCR和巢式PCR检测中诺达病毒均为阴性。巢式PCR阳性结果来自以下物种：双斑鲟、南方驼背虾和地中海贻贝。基于RNA2衣壳蛋白基因部分核苷酸序列（177个碱基）的系统发育分析表明，这四个毒株高度同源（100%），且与已知的β-诺达病毒、红斑石斑鱼神经坏死病毒（RGNNV）密切相关。这些结果表明，诺达病毒存在于朝鲜半岛南部沿海地区的野生海洋无脊椎动物中。这些亚临床感染的海洋无脊椎动物可能构成β-诺达病毒感染的接种源，并导致韩国养殖鱼类死亡。
