双斑蟳（米尔恩-爱德华兹，1886年）线粒体全基因组的特征分析
Characterization of complete mitochondrial genome of two-spot swimming crab (Miers, 1886).
作者信息
Kim Nack-Keun, Andriyono Sapto, Kim Ah Ran, Lee Chung Il, Kim Hyun-Woo
机构信息
Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University, Busan, Republic of Korea.
Department of Marine, Fisheries and Marine Faculty, Universitas Airlangga C Campus Jl, Mulyorejo Surabaya East Java, Indonesia.
出版信息
Mitochondrial DNA B Resour. 2018 Aug 17;3(2):900-901. doi: 10.1080/23802359.2018.1501292.
The two-spot swimming crab (Miers, 1886) is an important decapod species in the benthic ecosystem of Korean waters. In this study, we determined its complete mitochondrial genome by the combination of NGS analysis using MiSeq platform and PCR-based cloning method. The circular mitochondrial genome of was 15,714 bp in length in which the standard set of 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes were encoded. Phylogenic analysis showed that is most closely related to . The complete mitogenome sequence information of would provide useful data for the conservation of their population in the Pacific ocean.
双斑梭子蟹（米尔恩斯，1886年）是韩国海域底栖生态系统中的一种重要十足目物种。在本研究中，我们通过使用MiSeq平台的NGS分析和基于PCR的克隆方法相结合，确定了其完整的线粒体基因组。该线粒体基因组呈环状，长度为15,714 bp，其中编码了13个标准的蛋白质编码基因、22个tRNA基因和2个rRNA基因。系统发育分析表明，该物种与[具体物种]关系最为密切。双斑梭子蟹完整的线粒体基因组序列信息将为太平洋地区其种群的保护提供有用的数据。
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