Mondalek Fadee G, Lawrence Benjamin J, Kropp Bradley P, Grady Brian P, Fung Kar-Ming, Madihally Sundar V, Lin Hsueh-Kung
Department of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, OK 73019, USA.
Biomaterials. 2008 Mar;29(9):1159-66. doi: 10.1016/j.biomaterials.2007.11.020.
Small intestinal submucosa (SIS) derived from porcine small intestine has been intensively studied for its capacity in repairing and regenerating wounded and dysfunctional tissues. However, SIS suffers from a large spectrum of heterogeneity in microarchitecture leading to inconsistent results. In this study, we introduced nanoparticles (NPs) to SIS with an intention of decreasing the heterogeneity and improving the consistency of this biomaterial. As determined by scanning electron microscopy and urea permeability, the optimum NP size was estimated to be between 200 nm and 500 nm using commercial monodisperse latex spheres. The concentration of NPs that is required to alter pore sizes of SIS as determined by urea permeability was estimated to be 1 mg/ml 260 nm poly(lactic-co-glycolic) acid (PLGA) NPs. The 1mg/ml PLGA NPs loaded in the SIS did not change the tensile properties of the unmodified SIS or even alter pH values in a cell culture environment. More importantly, PLGA NP modified SIS did not affect human mammary endothelial cells (HMEC-1) morphology or adhesion, but actually enhanced HEMC-1 cell growth.
源自猪小肠的小肠黏膜下层（SIS）因其修复和再生受伤及功能失调组织的能力而受到广泛研究。然而，SIS在微观结构上存在很大的异质性，导致结果不一致。在本研究中，我们将纳米颗粒（NPs）引入SIS，旨在降低这种异质性并提高这种生物材料的一致性。通过扫描电子显微镜和尿素渗透性测定，使用商业单分散乳胶球估计最佳NP尺寸在200纳米至500纳米之间。根据尿素渗透性测定，改变SIS孔径所需的NP浓度估计为1毫克/毫升260纳米聚乳酸-乙醇酸共聚物（PLGA）NP。加载在SIS中的1毫克/毫升PLGA NP不会改变未修饰SIS的拉伸性能，甚至不会在细胞培养环境中改变pH值。更重要的是，PLGA NP修饰的SIS不会影响人乳腺内皮细胞（HMEC-1）的形态或黏附，但实际上会促进HEMC-1细胞生长。
