Zhang Hong-ye, Lu Jin-chun, Zhang Rui-sheng, Xia Yong-xiang, Huang Yu-feng
Department of Laboratory Science, the First Hospital of Nanjing Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210006, China.
Zhonghua Nan Ke Xue. 2007 Nov;13(11):1016-9.
To establish the method of seminal uric acid (UA) determination and investigate the correlation between the seminal UA level and semen parameters.
The method of seminal UA determination was established by modifying the method of serum UA detection, and its intraassay coefficient of variation (CV) and the difference of the results between different technicians were also observed to evaluate the acceptability of the method. In the meanwhile, the correlations of the seminal UA level with the patients' age, abstinence time, semen volume, pH, sperm concentration, motility, the percentage of grade a and b sperm, and the percentage of morphologically normal sperm.
The intraassay CV was 9. 16% for the method of seminal UA detection, and there was no significant difference in the UA level detected by 2 technicians (P = 0.541). The seminal UA level was positively correlated with the percentage of morphologically normal sperm (r = 0.350, P = 0.025) , with a tendency of positive correlation with sperm motility (r = 0.147, P = 0.085) and the percentage of grade a and b sperm (r = 0.156, P = 0.068), but not with other parameters such as semen volume, pH, sperm concentration, abstinence time and the patients' age.
An acceptable method of seminal UA determination could be established by modifying the method of serum UA detection. Sperm morphology, motility and the percentage of grade a and b sperm might be related to the level of seminal UA.
建立精液尿酸（UA）测定方法，并探讨精液UA水平与精液参数之间的相关性。
通过改良血清UA检测方法建立精液UA测定方法，并观察其批内变异系数（CV）以及不同技术人员检测结果的差异，以评估该方法的可接受性。同时，分析精液UA水平与患者年龄、禁欲时间、精液量、pH值、精子浓度、活力、a级和b级精子百分比以及形态正常精子百分比之间的相关性。
精液UA检测方法的批内CV为9.16%，两名技术人员检测的UA水平差异无统计学意义（P = 0.541）。精液UA水平与形态正常精子百分比呈正相关（r = 0.350，P = 0.025），与精子活力（r = 0.147，P = 0.085）和a级和b级精子百分比呈正相关趋势（r = 0.156，P = 0.068），但与精液量、pH值、精子浓度、禁欲时间和患者年龄等其他参数无关。
通过改良血清UA检测方法可建立可接受的精液UA测定方法。精子形态、活力以及a级和b级精子百分比可能与精液UA水平有关。
