Xu H-R, Lu J-C, Chen F, Huang Y-F, Yao B, Lu N-Q
Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, PR China.
Arch Androl. 2006 Nov-Dec;52(6):441-6. doi: 10.1080/01485010600822689.
To evaluate the effect of chymotrypsin on the examination of alpha-glucosidase activity in seminal plasma, thirty-nine samples of fresh liquefied semen with or without chymotrypsin and forty-eight samples of fresh un-liquefied semen with chymotrypsin were determined for the total alpha-glucosidase activity in seminal plasma. The total alpha-glucosidase level of each sample was assayed by the method of glucose oxidase. The correlations between alpha-glucosidase level and semen parameters, including semen volume, pH, sperm concentration, grade a and b motility and total motility, were analyzed with SPSS 11.0 software. The results showed that chymotrypsin had no effect on seminal alpha-glucosidase activity determination. Chymotrypsin could improve the liquefaction for un-liquefied semen, and there was no significant difference of alpha-glucosidase activity between liquefied and un-liquefied semen samples. There were significantly positive correlations between seminal alpha-glucosidase activity (U/ml) and sperm concentration (r = 0.338, p = 0.015) and between total alpha-glucosidase activity (U/ejaculate) and semen volume (r = 0.677, p = 0.000). However, there was no significant correlation between alpha-glucosidase level (U/ml) and semen volume, pH, sperm motility or grade a and b motility (r = -0.234 approximately 0.077, p = 0.099 approximately 0.993). The data indicated that chymotrypsin could be added into the un-liquefied semen samples for alpha-glucosidase activity determination, and there were different correlations between seminal alpha-glucosidase level and various semen parameters.
为评估糜蛋白酶对精浆中α-葡萄糖苷酶活性检测的影响，对39份添加或未添加糜蛋白酶的新鲜液化精液样本以及48份添加糜蛋白酶的新鲜未液化精液样本进行精浆总α-葡萄糖苷酶活性测定。采用葡萄糖氧化酶法检测各样本的总α-葡萄糖苷酶水平。运用SPSS 11.0软件分析α-葡萄糖苷酶水平与精液参数（包括精液体积、pH值、精子浓度、a级和b级活力以及总活力）之间的相关性。结果显示，糜蛋白酶对精浆α-葡萄糖苷酶活性测定无影响。糜蛋白酶可改善未液化精液的液化情况，且液化与未液化精液样本之间的α-葡萄糖苷酶活性无显著差异。精浆α-葡萄糖苷酶活性（U/ml）与精子浓度（r = 0.338，p = 0.015）以及总α-葡萄糖苷酶活性（U/射精量）与精液体积（r = 0.677，p = 0.000）之间存在显著正相关。然而，α-葡萄糖苷酶水平（U/ml）与精液体积、pH值、精子活力或a级和b级活力之间无显著相关性（r = -0.234至0.077，p = 0.099至0.993）。数据表明，在未液化精液样本中添加糜蛋白酶可用于α-葡萄糖苷酶活性测定，且精浆α-葡萄糖苷酶水平与各种精液参数之间存在不同的相关性。
