Cheng Po-Ching, Hsu Che-Yuan, Chen Chin-Chu, Lee Kin-Mu
Institute of Tropical Medicine, National Yang-Ming University, Taipei, Taiwan.
Toxicol Appl Pharmacol. 2008 Mar 1;227(2):291-8. doi: 10.1016/j.taap.2007.10.023. Epub 2007 Nov 7.
Antrodia camphorata (A. camphorata) is a fungus commonly used for treatment of viral hepatitis and cancer in Chinese folk medicine. Extract of A. camphorate is reported to possess anti-inflammatory, antihepatitis B virus and anticancer activities. In this study, we tested the in vivo effects of polysaccharides derived from A. camphorata (AC-PS) on immune function by detection of cytokine expression and evaluation of the immune phenotype in a T1/T2 doubly transgenic mouse model. The protective effect of AC-PS in mice was tested by infection with Schistosoma mansoni. The induction of large amounts of IFN-gamma, IL-2 and TNF-alpha mRNA were detected after 2 and 4 weeks of oral AC-PS administration in BALB/c and C57BL/6 mice. In transgenic mice, 3 to 6 weeks of oral AC-PS administration increased the proportion of CD4(+) T cells and B cells within the spleen. More specifically, there was an increase of Th1 CD4(+) T cells and Be1 cells among spleen cells as observed by detection the of Type1/Type2 marker molecules. By using a disease model of parasitic infection, we found that AC-PS treatment inhibited infection with S. mansoni in BALB/C and C57BL/6 mice. AC-PS appears to influence the immune system of mice into developing Th1 responses and have potential for preventing infection with S. mansoni.
樟芝是一种在中国民间医学中常用于治疗病毒性肝炎和癌症的真菌。据报道，樟芝提取物具有抗炎、抗乙肝病毒和抗癌活性。在本研究中，我们通过检测细胞因子表达和评估T1/T2双转基因小鼠模型中的免疫表型，测试了樟芝多糖（AC-PS）对免疫功能的体内作用。通过用曼氏血吸虫感染来测试AC-PS对小鼠的保护作用。在BALB/c和C57BL/6小鼠口服AC-PS 2周和4周后，检测到大量IFN-γ、IL-2和TNF-α mRNA的诱导。在转基因小鼠中，口服AC-PS 3至6周增加了脾脏内CD4(+) T细胞和B细胞的比例。更具体地说，通过检测1型/2型标记分子，观察到脾细胞中Th1 CD4(+) T细胞和Be1细胞增加。通过使用寄生虫感染疾病模型，我们发现AC-PS处理抑制了BALB/C和C57BL/6小鼠的曼氏血吸虫感染。AC-PS似乎影响小鼠免疫系统产生Th1反应，并具有预防曼氏血吸虫感染的潜力。
