Chen Yu-Jen, Cheng Po-Ching, Lin Ching-Nan, Liao Hui-Fen, Chen Yu-Yawn, Chen Chin-Chu, Lee Kin-Mu
Department of Radiation Oncology, Mackay Memorial Hospital, Taipei, Taiwan.
Int Immunopharmacol. 2008 Mar;8(3):458-67. doi: 10.1016/j.intimp.2007.11.008. Epub 2007 Dec 28.
Antrodia camphorata (AC) is a commonly used fungus in folk medicine for the treatment of viral hepatitis and cancer. AC polysaccharides (AC-PS) are reported to possess anti-inflammatory, anti-hepatitis B virus, and anticancer activities. In this study, we tested the in vivo effect of AC-PS on immune function by evaluating cytokine expression; on immunomodulation, by evaluating spleen cells; and on Schistosoma mansoni infection in mice. The induction of high levels of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) mRNA was detected in BALB/c mice after 2, 4, and 6 weeks of oral AC-PS administration. After 6 weeks of oral AC-PS administration to the BALB/c mice, the number of splenic dendritic cells, macrophages, and the surface expression of CD8 alpha+ and major histocompatibility class II I-A/I-E on dendritic cells increased. The CD4+/CD8+ ratio and number of B cells among splenocytes were also augmented. By using a disease model of parasitic infection, we found that AC-PS treatment inhibited S. mansoni infection in BALB/c mice. AC-PS appears to modulate the immune system of mice and has potential for preventing S. mansoni infection.
樟芝是一种民间医学中常用于治疗病毒性肝炎和癌症的真菌。据报道，樟芝多糖（AC-PS）具有抗炎、抗乙肝病毒和抗癌活性。在本研究中，我们通过评估细胞因子表达来测试AC-PS对免疫功能的体内作用；通过评估脾细胞来测试其对免疫调节的作用；并测试其对小鼠曼氏血吸虫感染的影响。在BALB/c小鼠口服AC-PS 2、4和6周后，检测到高水平的干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）mRNA的诱导。在BALB/c小鼠口服AC-PS 6周后，脾树突状细胞、巨噬细胞的数量以及树突状细胞上CD8α+和主要组织相容性复合体II类I-A/I-E的表面表达增加。脾细胞中B细胞的CD4+/CD8+比值和数量也增加。通过使用寄生虫感染疾病模型，我们发现AC-PS治疗可抑制BALB/c小鼠的曼氏血吸虫感染。AC-PS似乎可调节小鼠的免疫系统，并具有预防曼氏血吸虫感染的潜力。
