Lammers P J, Kerr B J, Honeyman M S, Stalder K, Dozier W A, Weber T E, Kidd M T, Bregendahl K
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Poult Sci. 2008 Jan;87(1):104-7. doi: 10.3382/ps.2007-00255.
An experiment was conducted with laying hens to determine the AME(n) value of crude glycerol, a coproduct of biodiesel production. Crude glycerol (87% glycerol, 9% water, 0.03% methanol, 1.26% Na, and 3,625 kcal/kg of gross energy) was obtained from a commercial biodiesel production facility (Ag Processing Inc., Sergeant Bluff, IA). A total of forty-eight 40-wk-old laying hens (Hy-Line W-36) were placed in metabolic cages (2 hens/ cage) and given free access to the experimental diets. A corn and soybean meal-based basal diet (18% CP, 2,875 kcal/kg of AME(n), 4.51% Ca, 0.51% nonphytate P) was formulated with 15% glucose.H(2)O and 1% Celite. Four dietary treatments were created by substituting 0, 5, 10, or 15% crude glycerol for glucose.H(2)O (3,640 kcal/kg of AME(n)). After 7 d of dietary adaptation, excreta were collected twice daily for 3 d, freeze-dried, and analyzed for contents of DM, Kjeldahl N, acid-insoluble ash, and gross energy. Egg production was recorded daily, and eggs were collected on d 7 and 8 of the experiment for calculation of egg mass (egg production x egg weight). Feed consumption was measured over the 10-d experimental period. Egg-production data were analyzed by ANOVA with 4 treatments and 6 replications in a completely randomized experimental design. The AME(n) value of crude glycerol was estimated as the slope of the linear relationship between the inclusion rate of dietary crude glycerol and the glucose-corrected AME(n) value of the experimental diets. No significant treatment effects (P > 0.1) were apparent for egg-production rate (93.0%), egg weight (56.1 g), egg mass (52.2 g/d), or feed consumption (104 g/d). Linear regression analysis (P < 0.001, r(2) = 0.92, n = 24) revealed that the AME(n) value of the crude glycerol used in this study was 3,805 +/- 238 kcal/kg (mean +/- SEM; as-is basis) for laying hens.
进行了一项以产蛋母鸡为对象的试验，以测定生物柴油生产的副产品粗甘油的氮校正表观代谢能（AME(n)）值。粗甘油（87%甘油、9%水、0.03%甲醇、1.26%钠，以及每千克含3625千卡总能）取自一家商业生物柴油生产厂（爱荷华州塞尔吉特布拉夫市的Ag Processing Inc.）。总共48只40周龄的产蛋母鸡（海兰W-36）被放入代谢笼（每笼2只母鸡），自由采食试验日粮。以玉米和豆粕为基础配制了一种基础日粮（粗蛋白含量18%，AME(n)为每千克2875千卡，钙含量4.51%，非植酸磷含量0.51%），其中含有15%的葡萄糖·H₂O和1%的硅藻土。通过用0%、5%、10%或15%的粗甘油替代葡萄糖·H₂O（AME(n)为每千克3640千卡），创建了四种日粮处理。经过7天的日粮适应期后，每天收集两次排泄物，持续3天，冻干后分析其中干物质、凯氏氮、酸不溶性灰分和总能的含量。每天记录产蛋量，并在试验的第7天和第8天收集鸡蛋，用于计算蛋重（产蛋量×蛋重）。在10天的试验期内测量采食量。产蛋数据采用完全随机试验设计，通过方差分析进行分析，有4种处理和6个重复。粗甘油的AME(n)值通过日粮中粗甘油的添加比例与试验日粮葡萄糖校正AME(n)值之间的线性关系斜率来估算。对于产蛋率（93.0%）、蛋重（56.1克）、蛋重（每天52.2克）或采食量（每天104克），未观察到显著的处理效应（P>0.1）。线性回归分析（P<0.001，r²=0.92，n=24）表明，本研究中使用的粗甘油对产蛋母鸡的AME(n)值为每千克3805±238千卡（均值±标准误；原样基础）。
