The Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Laboratory of Animal Nutrition Metabolism, Jilin Academy of Agricultural Sciences Gongzhuling, Jilin 136100, China.
Poult Sci. 2020 Aug;99(8):3914-3920. doi: 10.1016/j.psj.2020.03.041. Epub 2020 Apr 25.
The apparent metabolizable energy (AME), AME corrected to zero-nitrogen retention (AMEn), and net energy (NE) values of 2 corn samples both stored for 3 yr were determined in laying hens with reference diet substitution method. Reference diet was formulated according to standard layer requirement, and test diets contained 50% of corn samples and 50% of the reference diet. Fifty-four Hy-Line Brown hens at the age of 36 wk were used. The heat production and energy metabolism of birds were measured in open-circuit respiratory chambers with 6 replicates (3 birds per replicate) per diet in a randomized design. Birds were fed experimental diets for 7 D in the chamber as adaptation. During the following 3 D, feed intake, metabolizable energy value, nitrogen balance, energy balance, egg production, O consumption, CO production, and energy efficiency were determined. The AME values of corn 1 and corn 2 were 3,485 and 3,675 kcal/kg DM, respectively. The corresponding AMEn values were 3,452 and 3,596 kcal/kg DM, and the NE values were 2,575 and 2,693 kcal/kg DM, respectively. The NE:AME ratios of corn 1 and corn 2 were 74.4 and 73.3%, respectively. The NE:AMEn ratios of corn 1 and corn 2 were 75.0 and 74.9%, respectively. The AME, AMEn, and NE values of the 2 corn samples both stored for 3 yr were lower than the literature values for fresh corn.
采用参照饲粮替代法测定了 2 种玉米样本在 3 年贮藏期内的表观代谢能(AME)、零氮保留校正的 AME(AMEn)和净能(NE)值。参照饲粮是根据标准产蛋鸡的营养需求配制的,试验饲粮含有 50%的玉米样本和 50%的参照饲粮。选用 54 只 36 周龄海兰褐蛋鸡,采用 6 个重复(每个重复 3 只鸡)的完全随机设计,在开放式呼吸测热室中测定鸡的产热量和能量代谢。鸡在代谢室中适应期内连续 7 d 喂饲试验饲粮,随后 3 d 内测定采食量、代谢能值、氮平衡、能量平衡、产蛋率、耗氧量、CO2 产量和能量效率。玉米 1 和玉米 2 的 AME 值分别为 3485 和 3675 kcal/kg DM,相应的 AMEn 值分别为 3452 和 3596 kcal/kg DM,NE 值分别为 2575 和 2693 kcal/kg DM。玉米 1 和玉米 2 的 NE:AME 比值分别为 74.4%和 73.3%。玉米 1 和玉米 2 的 NE:AMEn 比值分别为 75.0%和 74.9%。2 种贮藏 3 年的玉米样本的 AME、AMEn 和 NE 值均低于新鲜玉米的文献值。