Teke Mustafa, Sezgintürk M Kemal, Dinçkaya Erhan, Telefoncu Azmi
Faculty of Science and Arts, Chemistry Department, Muğla University, Muğla, Türkiye.
Prep Biochem Biotechnol. 2008;38(1):51-60. doi: 10.1080/10826060701774346.
Interest in molecular imprinted polymer techniques has increased because they allows for the improvement of some stability characteristics of enzymes. The high stability of molecularly imprinted enzymes for a substrate can make them ideal alternatives as recognition elements for sensors. A bioimprinted mushroom tissue homogenate biosensor was constructed in a very simple way. For this purpose, sulfite was used. The enzyme, polyphenol oxidase, was first complexed by using a competitive inhibitor, sulfite, in aqueous medium and then the enzyme was immobilized on gelatin by crosslinking with glutaraldehyde on a glass electrode surface. Similarly, polyphenol oxidase uncomplexed with sulfite was also immobilized on a glass electrode in the same conditions. The aim of the study was to compare the two biosensors in terms of their repeatability and thermal, pH, and operational stability; also, the linear ranges of the two biosensors were compared with each other.
对分子印迹聚合物技术的兴趣有所增加，因为它们能够改善酶的某些稳定性特征。分子印迹酶对底物的高稳定性使其成为传感器识别元件的理想替代品。一种生物印迹蘑菇组织匀浆生物传感器的构建方法非常简单。为此，使用了亚硫酸盐。首先在水性介质中使用竞争性抑制剂亚硫酸盐使多酚氧化酶形成复合物，然后通过在玻璃电极表面与戊二醛交联将该酶固定在明胶上。同样，未与亚硫酸盐形成复合物的多酚氧化酶也在相同条件下固定在玻璃电极上。该研究的目的是比较这两种生物传感器在重复性、热稳定性、pH稳定性和操作稳定性方面的差异；此外，还比较了这两种生物传感器的线性范围。
