Ozcan Hakki Mevlut, Sagiroglu Ayten
Trakya University, Faculty of Science, Department of Chemistry, Edirne, Turkey.
Artif Cells Blood Substit Immobil Biotechnol. 2010 Aug;38(4):208-14. doi: 10.3109/10731191003776744.
In this study the biosensor was constructed by immobilizing tissue homogenate of banana peel onto a glassy carbon electrode surface. Effects of immobilization materials amounts, effects of pH, buffer concentration and temperature on biosensor response were studied. In addition, the detection ranges of 13 phenolic compounds were obtained with the help of the calibration graphs. Storage stability, repeatability of the biosensor, inhibitory effect and sample applications were also investigated. A typical calibration curve for the sensor revealed a linear range of 10-80 microM catechol. In reproducibility studies, variation coefficient and standard deviation were calculated as 2.69%, 1.44 x 10(-3) microM, respectively.
在本研究中，通过将香蕉皮组织匀浆固定在玻碳电极表面构建了生物传感器。研究了固定化材料用量、pH值、缓冲液浓度和温度对生物传感器响应的影响。此外，借助校准曲线获得了13种酚类化合物的检测范围。还研究了生物传感器的储存稳定性、重复性、抑制作用及样品应用。该传感器的典型校准曲线显示儿茶酚的线性范围为10 - 80微摩尔。在重现性研究中，变异系数和标准偏差分别计算为2.69%、1.44×10⁻³微摩尔。
