Altug Cagrı, Mengulluoglu Umut, Kurt Elif, Kaya Secil, Dinckaya Erhan
Ege University, Biochemistry Department, Bornova-Izmir, Turkey.
Artif Cells Blood Substit Immobil Biotechnol. 2011 Oct;39(5):298-303. doi: 10.3109/10731199.2011.574635. Epub 2011 May 17.
A glucose oxidase-based biosensor was developed for the determination of α-amylase activity. The determination method is based on monitoring the decrease in dissolved oxygen concentration related to the starch concentration, for which starch gives a reaction with α-amylase. Optimization parameters, including glucose oxidase amount, gelatin amount, and glutaraldehyde percentage for cross-linking, were investigated. The effects of pH, buffer system, and temperature on the biosensor system were also investigated. The biosensor had a linear relation to α-amylase activity and good measurement correlation between 0.66 and 9.83 U/ml. In sample analysis studies, α-amylase activity in baker's yeast was determined by the biosensor.
开发了一种基于葡萄糖氧化酶的生物传感器用于测定α-淀粉酶活性。该测定方法基于监测与淀粉浓度相关的溶解氧浓度的降低，淀粉会与α-淀粉酶发生反应。研究了优化参数，包括葡萄糖氧化酶用量、明胶用量和交联用戊二醛百分比。还研究了pH、缓冲体系和温度对生物传感器系统的影响。该生物传感器与α-淀粉酶活性呈线性关系，在0.66至9.83 U/ml之间具有良好的测量相关性。在样品分析研究中，通过该生物传感器测定了面包酵母中的α-淀粉酶活性。
