槟榔（槟榔属植物）中原花青素的定性和定量高效液相色谱/质谱测定

Qualitative and quantitative HPLC/MS determination of proanthocyanidins in areca nut (Areca catechu).

作者信息

Wu Qingli, Yang Yonghong, Simon James E

机构信息

New Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Cook College, Rutgers University, 59 Dudley Road, New Brunswick, New Jersey 08901, USA.

出版信息

Chem Biodivers. 2007 Dec;4(12):2817-26. doi: 10.1002/cbdv.200790231.

DOI:10.1002/cbdv.200790231

PMID:18081092

Abstract

Proanthocyanidins (PACs) in areca nut (Areca catechu L.) were analyzed by high-performance liquid chromatography (HPLC) combined with electrospray-ionization mass spectrometry (ESI-MS) and compared to grape seed extract. Under optimized conditions, the separated PACs were individually analyzed and identified on the basis of their [M+H]+ peaks. The PAC distribution in areca nut was found to be very similar to that in grape seed, but lacking any gallate conjugates. Based on reverse-phase HPLC separation, the PAC monomers (+)-catechin (CA, 1) and (-)-epicatechin (EC; 2) were successfully quantified by ESI-MS in the selected-ion-monitoring (SIM) mode, (-)-epigallocatechin (EGC; 3) being used as internal standard. Detailed quality and validation assays showed that the accuracy and repeatability (n = 8) were within 10% for each analyte.

摘要

采用高效液相色谱（HPLC）结合电喷雾电离质谱（ESI-MS）对槟榔（Areca catechu L.）中的原花青素（PACs）进行分析，并与葡萄籽提取物进行比较。在优化条件下，对分离得到的PACs根据其[M+H]+峰进行单独分析和鉴定。结果发现，槟榔中PACs的分布与葡萄籽中的非常相似，但不存在任何没食子酸酯共轭物。基于反相HPLC分离，通过ESI-MS在选择离子监测（SIM）模式下成功定量了PAC单体（+）-儿茶素（CA，1）和（-）-表儿茶素（EC；2），（-）-表没食子儿茶素（EGC；3）用作内标。详细的质量和验证分析表明，每种分析物的准确度和重复性（n = 8）均在10%以内。