采用液相色谱/电喷雾电离-质谱法测定儿茶中主要儿茶素的含量。

Determination of the predominant catechins in Acacia catechu by liquid chromatography/electrospray ionization-mass spectrometry.

作者信息

Shen Diandian, Wu Qingli, Wang Mingfu, Yang Yonghong, Lavoie Edmond J, Simon James E

机构信息

Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey 08854, USA.

出版信息

J Agric Food Chem. 2006 May 3;54(9):3219-24. doi: 10.1021/jf0531499.

DOI:10.1021/jf0531499

PMID:16637676

Abstract

A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI-MS) method under selected ion monitoring mode (SIM) was developed to quantitate the predominant catechins, catechin, epicatechin, epicatechin-3-O-gallate, and epigallocatechin-3-O-gallate, in the medicinal plant catechu (Acacia catechu). Other major secondary products including caffeine, flavanol dimers, and flavonol glycosides were also identified by their molecular ion peaks and fragmentation peaks using LC/MS and LC/MS/MS. For the investigated ion concentration ranges of catechin, epicatechin, epicatechin-3-O-gallate, and epigallocatechin-3-O-gallate, good linearities (r2 > 0.99) were obtained for each calibration curve. Validation for this method showed an accuracy ranging from 1.06 to 11.76%, and the precision (relative standard deviation) varied between 1.60 and 9.36% for these four analytes. This is the first quantitative determination of all predominant catechins in catechu heartwood and leaves.

摘要

建立了一种在选择离子监测模式（SIM）下的高效液相色谱-电喷雾电离质谱联用（LC/ESI-MS）方法，用于定量测定药用植物儿茶（儿茶属儿茶）中主要的儿茶素，即儿茶素、表儿茶素、表儿茶素-3-O-没食子酸酯和表没食子儿茶素-3-O-没食子酸酯。还通过LC/MS和LC/MS/MS的分子离子峰和碎片峰鉴定了其他主要次生产物，包括咖啡因、黄烷醇二聚体和黄酮醇糖苷。对于儿茶素、表儿茶素、表儿茶素-3-O-没食子酸酯和表没食子儿茶素-3-O-没食子酸酯所研究的离子浓度范围，每条校准曲线均具有良好的线性（r2>0.99）。该方法的验证表明，这四种分析物的准确度在1.06%至11.76%之间，精密度（相对标准偏差）在1.60%至9.36%之间。这是首次对儿茶心材和叶片中所有主要儿茶素进行定量测定。